Fluorescent Cell Line
Fluorescent Cell Line - Related Videos
05:02
Cell Electrofusion Visualized with Fluorescence Microscopy
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Cited by 11 •
2010
In this video we demonstrate efficient electrofusion of cells in vitro by means of modified adherence method using electroporation and the subsequent detection of fused cells visualization with fluorescence microscopy.
06:42
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Cited by 32 •
2013
We review our recent results on the integration of fluorescent microscopy and imaging flow cytometry tools on a cell-phone using compact and cost-effective opto-fluidic attachments. These cell-phone based micro-analysis devices might be useful for cytometric analysis, such as performing various cell counting tasks as well as for high-throughput screening of e.g., water samples in resource limited settings.
19:16
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
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Cited by 8 •
2009
Selection, microinjection, and imaging of fluorescently-labeled F-actin via fluorescent speckle microscopy (FSM).
15:10
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
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Cited by 18 •
2014
Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy setups.
13:35
Fluorescence Activated Cell Sorting of Plant Protoplasts
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Cited by 92 •
2010
A method for isolating specific cell types from plant material is demonstrated. This technique employs transgenic marker lines expressing fluorescent proteins in particular cell types, cellular dissociation and Fluorescence Activated Cell Sorting. Additionally, a growth setup is established here that facilitates treatment of Arabidopsis thaliana seedlings prior to cell sorting.
FAQs
Fluorescent proteins absorb light at a suitable excitation wavelength and then emit light at a longer wavelength. This difference allows researchers to distinguish the protein’s signal from the incoming illumination and detect labeled cells or structures with imaging equipment. The resulting signal provides a visual readout that can be followed during cellular experiments.
The excitation wavelength supplies the energy needed to activate the fluorescent protein, while the longer emission wavelength produces the detectable signal. Matching illumination to these properties is therefore essential for observing labeled cells effectively. This optical separation supports visualization of cellular structures, processes, or selected cell populations without relying on the same wavelength for activation and detection.
Selection enriches for cells that retain the introduced fluorescent-protein gene and continue producing the marker. Stable expression makes the labeled population more consistent across observations, which is important for experiments that follow cells over time. It also supports repeated imaging and comparisons of cellular behavior, gene expression, or treatment responses within the same experimental system.
Because fluorescence can be detected by illuminating the cells and collecting emitted light, researchers can observe cellular behavior without needing to destroy the population for every measurement. Repeated imaging can reveal changes in cell location, structure, or behavior over time. This longitudinal approach is useful for tracking dynamic responses during signaling, differentiation, or treatment experiments.
A typical workflow begins by introducing a gene encoding a fluorescent protein into the cells. Researchers then select cells that stably express the introduced gene and use the resulting population for fluorescence-based observation or assays. The selected cells can subsequently support live-cell imaging, cell tracking, gene-expression studies, or high-throughput measurements, depending on the experimental goal.
These cell lines are useful when investigators need to visualize cellular structures, follow specific cell populations, or monitor behavior during an experiment. They also support studies of signaling, differentiation, disease mechanisms, and responses to experimental treatments. Their fluorescence enables observations in live cells and can contribute to high-throughput assays that examine many samples systematically.
Fluorescent cell lines can provide visual or measurable information about where cells are, how they behave, and how selected processes change under experimental conditions. In biology, researchers can apply them to gene-expression studies, cell tracking, and analyses of signaling or differentiation. They can also help evaluate treatment responses and investigate cellular changes associated with disease mechanisms.