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February 18, 2010
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A method for isolating specific cell types from plant material is demonstrated. This technique employs transgenic marker lines expressing fluorescent proteins in particular cell types, cellular dissociation and Fluorescence Activated Cell Sorting. Additionally, a growth setup is established here that facilitates treatment of Arabidopsis thaliana seedlings prior to cell sorting.
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Cite this Article
Bargmann, B. O. R., Birnbaum, K. D. Fluorescence Activated Cell Sorting of Plant Protoplasts. J. Vis. Exp. (36), e1673, doi:10.3791/1673 (2010).
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