Journal
/
/
Fluorescence Activated Cell Sorting of Plant Protoplasts
Fluorescence Activated Cell Sorting of Plant Protoplasts
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

24,686 Views

13:35 min

February 18, 2010

DOI:

13:35 min
February 18, 2010

5 Views
,

Summary

Automatically generated

A method for isolating specific cell types from plant material is demonstrated. This technique employs transgenic marker lines expressing fluorescent proteins in particular cell types, cellular dissociation and Fluorescence Activated Cell Sorting. Additionally, a growth setup is established here that facilitates treatment of Arabidopsis thaliana seedlings prior to cell sorting.

Related Videos

Read Article