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JoVE Journal Bioengineering
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
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From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Article DOI: 10.3791/51994 • 15:10 min • October 9th, 2014
October 9th, 2014 •
1,2, 3, 1, 2
1NEST Laboratory, Scuola Normale Superiore, 2Center for Nanotechnology Innovation, Instituto Italiano di Tecnologia, 3Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine

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Summary
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Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy setups.

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Fast Fluorescence Imaging Molecular Diffusion Law Live Cell Membranes Commercial Microscope Spatial Distribution Motion Membrane Components Lipids Proteins Cellular Functions Spatio-temporal Resolution Real Behavior Of Molecules Experimental Protocol Fluorescently-labeled Plasma-membrane Proteins Lipids High Spatiotemporal Resolution Population Behavior Given Region Of The Membrane Autocorrelation Function Time Delays Particle Movement Diffusion Protein Mean Square Displacement Imaging (iMSD) Apparent Diffusivity Average Displacement
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