Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy setups.
Di Rienzo, C., Gratton, E., Beltram, F., Cardarelli, F. From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope. J. Vis. Exp. (92), e51994, doi:10.3791/51994 (2014).