Fluorescent Dye Tracking
Fluorescent Dye Tracking - Related Videos
07:31
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
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Cited by 2 •
2017
This protocol describes an efficient way to monitor the cell persistence and biodistribution of human adipose-derived mesenchymal stem cells (haMSCs) by far-red fluorescence labeling in a rat knee osteoarthritis (KOA) model via intra-articular (IA) injection.
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
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Cited by 45 •
2012
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
02:42
Analyzing Synaptic Vesicle Recycling and Fluorescent Dye Uptake in Drosophila Larvae
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2025
This video demonstrates a protocol to study synaptic vesicle recycling at neuromuscular junctions in channelrhodopsin-expressing Drosophila larvae using a fluorescent dye. Blue light activates the channelrhodopsins, causing calcium influx and synaptic vesicle exocytosis. The dye integrates into the neuronal membranes and is internalized during endocytosis, resulting in enhanced fluorescence. Further blue light stimulation induces exocytosis and dye release, which is shown by reduced...
08:39
Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
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2025
This article provides a detailed description of how to create samples for single-protein tracking in solid-supported lipid bilayers. It also explains a straightforward fluorescence microscope with single-molecule sensitivity and a fast frame rate. Finally, we outline the procedure for extracting single-protein trajectories.
05:09
Delivery of a Fluorescent Dye into the Subretinal Space of a Mouse
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2025
Source: Parikh, S., et al. An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach. J. Vis. Exp. (2016)This video demonstrates the procedure for delivering a fluorescent dye into the subretinal space of a mouse. The process involves anesthetizing the mouse, preparing the eye, making precise incisions to access the subretinal space, and injecting the dye to create a controlled retinal detachment. This technique allows for targeted...
FAQs
The dye absorbs light at an appropriate excitation wavelength and emits light at a longer wavelength. This separation allows a fluorescence microscope to distinguish signal from the illuminating light and from surrounding material. As a result, labeled cells, molecules, or structures can be localized more clearly, supporting observation of their position and movement over time.
Attaching a label connects the light-emitting marker to a selected target, whereas introducing one places the label within the biological system for observation. This distinction influences whether the experiment follows a specific molecule, a cell, or a broader structure. The chosen strategy should match the biological feature and movement researchers need to examine.
A single image records where labeled material appears at one moment, while time-lapse imaging reveals changes across successive observations. Researchers can therefore examine movement, transport, migration, or changing tissue organization rather than only a static distribution. Quantitative analysis of these image sequences can make dynamic biological processes easier to compare and interpret.
A typical workflow introduces or attaches the light-emitting label, places the sample under a fluorescence microscope, and illuminates it at the appropriate excitation wavelength. The emitted signal is then recorded, either in individual images or across a time series. Researchers analyze the labeled locations, movements, or interactions to obtain the intended biological measurement.
The central instrument is a fluorescence microscope capable of illuminating the dye at its appropriate excitation wavelength and detecting the emitted light. Imaging may be organized as a time series when movement or transport is the focus. Consistent acquisition conditions help researchers distinguish labeled targets from surrounding material and support meaningful quantitative comparisons.
This approach is useful when researchers need to follow cell migration, intracellular transport, molecular uptake, or changes in tissue organization. Its time-resolved measurements can support investigations of development, physiology, disease, and therapeutic response. By linking fluorescent signal with location and change over time, the method provides evidence about dynamic biological behavior rather than only fixed structure.