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In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
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JoVE Journal Developmental Biology
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

DOI:
10.3791/56273-v

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07:31 min

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October 08, 2017

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, , , ,
1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics,Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital,Tongji University

Chapters

  • 00:05Title
  • 00:58Surgical Rat Knee Osteoarthritis (KOA) Induction
  • 02:36Human Mesenchymal Stem Cell Fluorescent Dye Labelling and In Vivo Fluorescent Imaging
  • 05:16Results: Representative Rat KOA Induction and haMSC Imaging Analyses
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes an efficient way to monitor the cell persistence and biodistribution of human adipose-derived mesenchymal stem cells (haMSCs) by far-red fluorescence labeling in a rat knee osteoarthritis (KOA) model via intra-articular (IA) injection.

Tags

Keywords: In Vivo TrackingHuman Adipose-derived Mesenchymal Stem CellsRat Knee Osteoarthritis ModelFluorescent Lipophilic Membrane DyeDiDStem Cell PersistenceBiodistributionRegenerative MedicineAnimal ModelCell LabelingIn Vivo Imaging

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