Genetic Over-expression Techniques
Genetic Over-expression Techniques - Related Videos
10:17
An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
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Cited by 2 •
2010
Genetic associations often remain unexplained at a functional level. This method aims to assess the effect of phenotype-associated genetic markers on gene expression by analyzing cells heterozygous for transcribed SNPs. The technology allows accurate measurement by MALDI-TOF mass spectrometry to quantify allele-specific primer extension products.
03:01
Detection of Target Enzyme Expression in Genetically Modified Bacterial Cells
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2025
Source: Kim, H. et. al., Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System. J. Vis. Exp. (2016)This video demonstrates a genetic screening method to detect enzyme expression using metagenomic fosmid libraries and chromogenic substrates. Flow cytometry identifies and isolates single cells producing colored products, enabling high-throughput selection of active enzyme-expressing cells.
10:31
The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
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Cited by 11 •
2016
This protocol demonstrates how to generate fluorescently marked, genetically defined clonal tumors in the Drosophila eye/antennal imaginal discs (EAD). It describes how to dissect the EAD and brain from the third instar larvae and how to process them to visualize and quantify gene expression changes and tumor invasiveness.
08:41
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
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Cited by 21 •
2018
Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.
10:27
Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
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Cited by 12 •
2011
Microarray analysis was conducted to determine genetic expression profiles in C. elegans, and real-time PCR was used to validate and quantify microarray data.
FAQs
Regulatory elements are included with the introduced gene to drive its transcription, thereby influencing how much gene-derived RNA is produced. Their role is central because elevated transcription provides the basis for increasing the corresponding gene product. In a biological experiment, this control helps researchers examine how greater gene activity affects cellular function, signaling pathways, or responses under defined conditions.
Transient delivery produces elevated expression for an experimental period without establishing the same lasting maintenance as integration. Stable integration is selected when researchers need the introduced gene to remain present and support sustained elevated expression under defined conditions. This distinction affects how scientists design studies in cultured cells or model organisms and how they assess resulting cellular effects.
Producing excess RNA or protein can amplify the activity or effects associated with a gene product. That increased level may expose a phenotype, cellular response, or signaling effect that is not apparent when the gene operates at its normal expression level. Consequently, the approach helps connect gene activity with observable biological outcomes in cells and model organisms.
A study generally begins by selecting the gene of interest and a suitable expression system, then pairing the gene with regulatory elements that drive transcription. Researchers choose either transient delivery or stable integration according to the intended expression pattern, introduce the construct into cultured cells or a model organism, and maintain the experiment under defined conditions for analysis.
These approaches are useful when researchers want to examine a gene product's function, activity, or effects after increasing its production. They support investigations of signaling pathways and cellular responses in cultured cells and model organisms. By creating elevated expression, experiments can reveal biological consequences that may remain unclear at normal expression levels.
In biotechnology, increased gene expression can support improved production of a desired protein. In therapeutic research, the same strategy can help validate therapeutic targets by testing the consequences of elevated gene-product activity. These applications extend the method beyond basic gene-function studies, linking controlled expression changes with protein production goals and evaluation of biologically relevant targets.