Human Lung Epithelial
Human Lung Epithelial - Related Videos
11:49
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
0 Views •
Cited by 17 •
2020
This article provides a detailed methodology for tissue dissociation and cellular fractionation approaches allowing enrichment of viable epithelial cells from proximal and distal regions of the human lung. Herein these approaches are applied for the functional analysis of lung epithelial progenitor cells through the use of 3D organoids culture models.
03:15
Analyzing the Effects of Cigarette Smoke on Antimicrobial Defense in Lung Epithelial Cells
0 Views •
2026
Source: Li, T., et al. Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells. J. Vis. Exp. (2020).This video demonstrates how cigarette smoke extract weakens antimicrobial defense in lung epithelial cells by reducing AMP production, leading to increased bacterial invasion. The procedure measures intracellular bacterial load to assess the impact of smoke exposure on host-pathogen interactions.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
0 Views •
Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
0 Views •
Cited by 135 •
2013
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.
12:31
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
0 Views •
Cited by 8 •
2016
This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.
FAQs
Its barrier function regulates contact between inhaled material, lung tissue, and the bloodstream while preserving the specialized conditions needed for gas exchange. Epithelial injury or altered barrier function can therefore affect both protection and communication across the lung interface. Studying these changes helps explain how inflammation, toxic exposures, and pulmonary diseases disrupt normal respiratory biology.
Mucus helps protect airway surfaces by trapping inhaled particles, while ciliary beating moves that material through the airway lining. Surfactant contributes to the protective and functional environment of the lung, particularly at epithelial surfaces involved in gas exchange. Examining these coordinated activities allows researchers to assess how epithelial cells maintain airway defense and lung stability.
Thin alveolar surfaces place epithelial cells close to the bloodstream, supporting efficient gas exchange across the air-lung interface. This arrangement also makes changes in epithelial integrity especially relevant to respiratory disease research. When injury or altered barrier function affects these surfaces, researchers can investigate how cellular damage may influence gas exchange and broader lung health.
These model types provide complementary ways to study human lung epithelial behavior. Primary cultures support investigation of epithelial cells in a laboratory setting, whereas organoids and engineered lung models provide additional systems for examining tissue organization and function. Comparing these approaches can help researchers select a model suited to studying injury, repair, disease mechanisms, or drug responses.
Researchers may use these models when they need to examine how lung epithelial tissue responds to a potential treatment. Primary cultures, organoids, and engineered systems can support testing related to respiratory infections, inflammation, toxic exposures, asthma, or pulmonary fibrosis. The resulting observations may help connect treatment responses with epithelial injury, repair, or altered barrier function.
This research can clarify how epithelial injury develops, how repair is studied, and how changes in barrier function relate to respiratory health. It also provides a framework for investigating infections, inflammation, toxic exposures, asthma, and pulmonary fibrosis. In biology, these questions connect cell behavior and tissue interfaces with clinically important changes in lung function.