Immunosuppressive Assay
Immunosuppressive Assay - Related Videos
08:38
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
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Cited by 28 •
2015
Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.
09:25
Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
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Cited by 7 •
2015
Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.
05:40
Establishment of A Mouse Sepsis-Induced Immunosuppression Model By Intranasal Instillation of Bacteria
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2026
Here, we present a protocol for establishing a mouse sepsis immunosuppression model via intranasal instillation of Klebsiella pneumoniae and validated the model through various indicators.
13:37
A Mouse Tumor Model of Surgical Stress to Explore the Mechanisms of Postoperative Immunosuppression and Evaluate Novel Perioperative Immunotherapies
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Cited by 33 •
2014
A mouse tumor model of surgical stress is used to explore how postoperative immune suppression promotes metastatic disease and to evaluate immunostimulatory perioperative therapies.
07:30
Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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Cited by 7 •
2019
This protocol describes techniques to measure infectious outcomes underlying secondary hospital-acquired infections in the immunosuppressive condition, first by establishing cecal ligation/puncture mice then challenging them with intranasal infection to create a clinically relevant model of immunosuppression sepsis.
FAQs
The readout determines which aspect of immune activity is being examined. Proliferation indicates whether stimulated lymphocytes continue expanding, cytokine production reflects changes in immune signaling, activation markers show altered cellular status, and viability indicates whether cells remain alive. Using these measurements helps characterize the nature and extent of a treatment’s suppressive effect.
Stimulation creates a measurable immune response against which suppression can be evaluated, while untreated controls show the response in the absence of the tested substance, cell population, or treatment. Comparing the two conditions allows researchers to attribute differences in proliferation, cytokine production, activation markers, or viability to the experimental intervention.
The assay can examine immune-function measurements alongside cell viability rather than relying on a single endpoint. A decrease in proliferation, cytokine production, or activation markers with maintained viability supports reduced immune activity. If viability also declines, the result may reflect loss of cells, making the suppressive effect more difficult to interpret.
Researchers can compare different substances, cell populations, or treatments and assess their effects using more than one immune readout. This approach reveals whether an intervention changes proliferation, cytokine production, activation markers, viability, or several of these features together. Such comparisons support a more complete characterization of immune suppression.
A basic workflow stimulates immune cells, exposes them to the substance, cell population, or treatment being examined, and measures selected indicators of immune activity. Results are then compared with untreated controls. The resulting differences provide measurable evidence for assessing suppression and for characterizing how the intervention affects the tested cells.
These assays are useful during screening and characterization of immunosuppressive drugs, biologics, and cellular therapies. By measuring changes in immune-cell proliferation, cytokine production, activation markers, or viability, researchers can compare candidate interventions and assess treatment potency. The measurements also provide evidence for whether a therapy produces the intended reduction in immune activity.
In biology research, immunosuppressive assays provide measurable data for investigating how treatments alter immune regulation. Their readouts can help evaluate potential effects on inflammatory or autoimmune responses by showing changes in activated immune-cell behavior and related outputs. This connects cellular measurements with broader questions about controlling excessive or unwanted immune activity.