Intestinal Uptake
Intestinal Uptake - Related Videos
05:59
Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
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Cited by 15 •
2011
M cells in a specialized follicle-associated epithelium covering Peyer’s patches play an important role for the mucosal immunosurveillance in gut-associated lymphoid tissue. Here we described the evaluation method for bacterial transcytosis by M cells in vivo. This method provides a method to understand M-cell function in the immune system.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
04:04
Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids
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2025
This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.
04:34
Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds
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Cited by 13 •
2018
The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics.
03:13
Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria
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2025
This video describes a plate reader-based assay to measure mitochondrial calcium uptake in isolated mitochondria. The assay analyzes the fluorescence signal of a calcium-sensitive fluorescent dye to study the kinetics of mitochondrial calcium uptake and calcium overload.
FAQs
Intestinal uptake can proceed through three broad routes: passive diffusion, transporter-mediated movement, or vesicular uptake. Passive diffusion reflects movement associated with molecular properties, whereas transporter-mediated movement depends on epithelial transport systems. Vesicular uptake uses cell-associated vesicles to move substances. Distinguishing these routes helps researchers explain why different substances cross the epithelium differently.
Efficiency reflects the substance’s molecular properties together with the epithelial barriers and local conditions at the intestinal interface. These variables can alter whether movement favors passive diffusion, transporter-mediated transfer, or vesicular uptake. Consequently, the same intestinal environment may not handle every nutrient, dietary compound, or orally delivered drug in the same way.
The destination matters because it determines how absorbed material connects the intestine with the rest of the body. Tracking whether a substance reaches the circulation or lymphatic system helps characterize its biological availability and supports interpretation of nutrient handling, drug delivery, and dietary-compound studies in biological research.
Changes in intestinal uptake can alter how much of a nutrient becomes available after crossing the epithelial barrier. Studying these differences helps connect epithelial transport with nutrient availability and metabolic regulation, while also showing how activity at the gut interface interacts with the rest of the body. This makes uptake a useful link between intestinal biology and broader physiology.
A useful comparison considers the substance being examined, the route by which it crosses the epithelium, the epithelial barrier, and relevant local conditions. Researchers can then relate observed movement to entry into the circulation or lymphatic system. This framework supports interpretation without treating all transported substances as biologically equivalent.
For oral drug delivery, uptake is relevant because transport across the intestinal epithelium contributes to bioavailability, meaning the availability of a substance to the body after delivery. Molecular properties, epithelial barriers, and local conditions can influence this process, so intestinal studies help explain why a delivered compound may not show the same biological availability under every intestinal condition.
Disease-related changes in epithelial transport can modify how nutrients or other substances become available to the body. Examining those changes helps connect intestinal biology with health outcomes and may clarify why treatment responses vary when oral drug delivery depends on passage across the intestinal epithelium. Altered uptake can therefore provide context for studying disease and therapy together.
The potential biological relevance of a dietary compound depends partly on whether it can move beyond the intestinal lumen. Studying its uptake helps relate diet-related exposure to nutrient availability, metabolic regulation, and interactions between the gut and the rest of the body. This perspective connects intestinal transport with broader questions about how dietary substances influence biology.