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Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds
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Immunology and Infection
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JoVE Journal Immunology and Infection
Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds

Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds

DOI:
10.3791/58366-v

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04:33 min

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September 27, 2018

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, ,
1Institute of Biotechnology and Biomedicine (IBB-UAB),Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology,Universitat Autònoma de Barcelona

Chapters

  • 00:04Title
  • 00:58Preparing the Equipment for Oral Intubation
  • 01:22Zebrafish Anesthetization and Oral Intubation
  • 02:40Zebrafish Intestine Dissection
  • 03:22Results: Home-made Oral Intubation Device Successfully Delivers Fluorescent Nanoparticles IBsTNFα with Low Average Percent Mortality
  • 04:01Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics.

Tags

Keywords: Oral IntubationZebrafishIntestinal UptakeBioactive CompoundsMucosa VaccinologyNanoparticle SuspensionIntestinal DissectionAnesthetizingRecovery TankEuthanizing

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