Lsr Two Flow Cytometer
Lsr Two Flow Cytometer - Related Videos
13:59
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
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Cited by 9 •
2014
Spaceflight blood diagnostics need innovation. Few demonstrations have been published illustrating in-flight, reduced-gravity health diagnostic technology. Here we present a method for construction and operation of a parabolic flight test rig for a prototype point-of-care flow-cytometry design, with components and preparation strategies adaptable to other setups.
10:58
Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
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Cited by 14 •
2011
This protocol describes how to perform cell viability and fluorescence expression assays using the Tali Image-Based Cytometer.
06:22
Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
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Cited by 4 •
2023
In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple centers.
11:37
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
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Cited by 6 •
2010
This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
FAQs
Hydrodynamic focusing organizes the cell-containing fluid into a narrow stream so individual cells pass through the laser-illuminated region in a controlled sequence. This alignment helps detectors associate scattered-light and fluorescence signals with particular cells rather than with a mixed population. As a result, the instrument can generate quantitative measurements for many individual cells.
Scattered light contributes information about a cell’s physical characteristics, while fluorescence reports labeled molecules associated with the cell. The LSR II combines these signal types to distinguish cellular populations and evaluate molecular differences. This separation is useful when researchers need to relate cell properties to protein expression or other labeled biological features.
Multiparameter analysis records several physical and fluorescent characteristics from the same individual cells. Rather than relying on a single measurement, researchers can compare multiple features to identify populations and examine variation within them. This approach is especially valuable for revealing biological heterogeneity, in which cells in one sample do not all share the same state or expression profile.
Fluorescent labels provide detectable signals from molecules associated with individual cells. During analysis, laser illumination excites the labeled molecules, and detectors capture the resulting fluorescence alongside scattered light. Researchers can then use the combined signals to assess protein expression and distinguish cellular groups with different molecular characteristics.
A measurement guides cells through a fluid stream, aligns them by hydrodynamic focusing, and exposes them to laser light one cell at a time. Detectors collect scattered-light and fluorescence signals, which are converted into multiparameter data. The resulting measurements support quantitative comparison of cell populations and cellular characteristics.
Researchers can apply the LSR II when they need quantitative information about cell populations, protein expression, immune responses, viability, or activation. In biology, these measurements support immunology and cell biology investigations, while broader uses include disease research and experimental medicine. Its single-cell analysis helps reveal differences that population-level averages could conceal.