Milliequivalents Per Liter

Milliequivalents per liter (mEq/L) is a concentration unit that expresses the chemical combining capacity of charged particles in a solution, accounting for both their amount and valence. It is calculated by multiplying a substance’s molar concentration by its ionic charge and converting the result to thousandths of an equivalent per liter, so a divalent ion contributes twice the equivalents of a monovalent ion at the same molar concentration. In biology, mEq/L is used to report electrolytes such as sodium, potassium, calcium, chloride, and bicarbonate in blood and other fluids. These measurements help assess osmotic balance, membrane excitability, acid–base status, and physiological disturbances.

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Research

JoVE Journal - Chemistry
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Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

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Cited by 17 •

2016

Channels for the transportation of water molecules in enzymes influence active site solvation and catalysis. Herein we present a protocol for the engineering of these additional catalytic motifs based on in silico computer modeling and experiments. This will enhance our understanding of the influence of solvent dynamics on enzyme catalysis.

Research

JoVE Journal - Medicine

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

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Cited by 31 •

2013

A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

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Cited by 18 •

2012

A method to analyse the distribution of bone marrow hematopoietic progenitors in flow cytometry as well as to efficiently isolate highly purified hematopoietic stem cells (HSCs) is described. The isolation procedure is essentially based on magnetic enrichment of c-Kit+ cells and cell sorting to purify HSCs for cellular and molecular studies.

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

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Cited by 1 •

2017

This is a time- and cost-effective in vitro protocol investigating the mechanisms of acquired resistance to targeted therapeutic agents, which is a highly unmet medical need in cancer management.

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

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Cited by 2 •

2019

We present a protocol to evaluate the expression levels of circulating microRNA (miRNAs) in plasma samples from cancer patients. In particular, we used a commercially available kit for circulating miRNA extraction and reverse transcription. Finally, we analyzed a panel of 24 selected miRNAs using real-time pre-spotted probe custom plates.

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