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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

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08:12 min

March 14, 2019

DOI:

08:12 min
March 14, 2019

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Summary

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We present a protocol to evaluate the expression levels of circulating microRNA (miRNAs) in plasma samples from cancer patients. In particular, we used a commercially available kit for circulating miRNA extraction and reverse transcription. Finally, we analyzed a panel of 24 selected miRNAs using real-time pre-spotted probe custom plates.

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