Mouse Blastocysts
Mouse Blastocysts - Related Videos
10:19
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
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Cited by 7 •
2010
In this video, we demonstrate the isolation of mouse blastocysts and the derivation of trophoblast stem cells from blastocysts. We also describe conditions for maintenance of the stem cell property as well as induction of differentiation in culture.
10:59
Human Blastocyst Biopsy and Vitrification
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Cited by 36 •
2019
Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.
12:09
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
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Cited by 6 •
2022
A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.
09:35
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
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Cited by 11 •
2017
MicroSecure Vitrification was developed as a non-commercial, aseptic closed vitrification device system that is compliant with FDA good manufacturing and tissue-handling practices. Due to the withdrawal of hydrophobic plugged embryo straws from the industry, the vitrification procedure was modified to include an inner seal before the standard internal cotton plug.
12:59
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
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Cited by 1 •
2017
This article describes a protocol to efficiently derive and culture pluripotent stem cell lines from mouse embryos at the blastocyst stage.
FAQs
Cleavage is accompanied by compaction, a process that brings embryonic cells into a more tightly organized structure. The embryo then develops a fluid-filled blastocoel, creating internal separation between the inner cell mass and the surrounding trophectoderm. These coordinated structural changes establish the arrangement needed for later differences in embryonic and extraembryonic contributions.
The inner cell mass provides the cells that form the embryo itself, whereas the trophectoderm surrounds it and contributes to extraembryonic tissues. This division reflects an early cell fate decision within the developing embryo. Studying these populations helps researchers examine how early mammalian cells become assigned to distinct developmental outcomes.
Hatching removes the zona pellucida that surrounds the preimplantation embryo, allowing the blastocyst to interact directly with the uterine lining. Following hatching, attachment to that lining initiates implantation. This sequence makes mouse blastocysts useful for investigating the transition from an independently enclosed embryo to one that establishes contact with maternal tissue.
Their separated inner cell mass and trophectoderm provide a defined context for examining early cell fate decisions. The inner cell mass is especially relevant to pluripotency, the capacity associated with forming the embryo, and to embryonic stem cell derivation. Consequently, these embryos support analysis of how developmental potential is maintained or directed during early mammalian development.
A typical developmental sequence follows cleavage, compaction, blastocoel formation, separation of the inner cell mass from the trophectoderm, hatching from the zona pellucida, and attachment to the uterine lining. Considering these stages in order allows researchers to connect changing embryo structure with cell fate, implantation, and the onset of interactions required for continued development.
Researchers use them when questions concern events immediately before implantation, including lineage allocation, embryo attachment, and genetic regulation of early development. They also serve as a model in reproductive science because their progression from a preimplantation structure to uterine attachment can be examined in relation to implantation and early mammalian development.
Studies can reveal how early cells acquire different developmental roles, how implantation begins after hatching, and how genetic regulation relates to these events. The model also supports embryonic stem cell derivation and investigation of pluripotency. Together, these applications connect cellular organization with broader questions in developmental biology, reproductive science, and mammalian embryo development.