Mouse Gut
Mouse Gut - Related Videos
01:55
Intracranial Injection of Gut-Derived Microbial Metabolites in a Mouse Model
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2025
Source: Liou, C. et al. Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice. J. Vis. Exp. (2022).This video demonstrates the intracranial injection of short-chain fatty acids (SCFAs) in a freely moving mouse to study their effects on brain-mediated behavior. An injector cannula is inserted into a guide cannula implanted in the mouse brain. After recovery, the mouse acclimates in a novel cage. SCFAs are then administered, and locomotor behavior is recorded.
04:32
Whole Mount Preparation of Mouse Intestine: Using a Simple Gut-incising Device to Prepare Whole Mount
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2023
In this video, we demonstrate whole mount preparation of the mouse intestine using a simple gut-incising device. The resulting mounts are of superior and uniform quality and can be used for histological analyses.
15:27
Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
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Cited by 18 •
2007
This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.
01:15
Assessing the Role of Gut Microbiota in Modulating Hepatitis B Infection in Mice
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2026
Take a control mouse with a normal gut microbiota and an experimental mouse with depleted gut microbiota. The depletion compromises the integrity of the gut barrier.This enables bacteria to translocate through the blood vessel into the liver tissue.Inject both mice with the hepatitis B virus or HBV plasmid to induce an HBV infection in the liver tissue.In the control mouse, the T cell detects an infected liver cell through the interaction of its receptors with viral antigens, and produces...
07:18
Oral Gavage in Neonatal Mouse Pups and Functional Assessment of Gut Barrier Integrity Using Ussing Chambers
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2026
This article describes: (1) oral gavage delivery of test molecules to neonatal mice as early as DOL 6; and (2) ex vivo evaluation of colonic epithelial barrier function in biopsies from DOL 10 to weaning (DOL 20) pups by assessing paracellular and transcellular permeability with Ussing chambers.
FAQs
Mechanical mixing, enzymatic digestion, epithelial nutrient transport, and microbial fermentation represent complementary processes in gut function. Mixing and digestion act on food within the tract, while epithelial transport determines how nutrients cross into host tissues. Fermentation adds a microbial contribution, making the mouse gut useful for examining how digestive and microbial activities operate together.
The intestinal epithelium has a dual role: it permits nutrient transport while helping limit exposure to gut contents. Immune functions add another layer of regulation by responding to that exposure. Studying these barrier and immune relationships in mice helps researchers investigate intestinal inflammation and understand how host tissues interact with material and microorganisms present in the gut.
Resident microorganisms participate in fermentation and provide a biological context for host-microbe interaction studies. In the mouse gut, researchers can consider microbial activity alongside epithelial, immune, and digestive processes rather than treating digestion as a host-only function. This integrated perspective supports work on intestinal biology and metabolic disease.
Its anatomy, physiology, microbiota, and immune responses can all be examined within a controlled experimental system. That combination allows investigators to connect changes in gut structure or function with host-microbe interactions, inflammation, diet, or therapeutics. The model therefore supports systematic study of several interacting features rather than one isolated process.
Researchers can use the mouse gut to investigate how dietary conditions relate to digestive activity, nutrient handling, microbial fermentation, and gut-associated immune or barrier functions. Because these features can be studied together, experiments can examine diet as a factor influencing multiple components of gastrointestinal biology rather than focusing on nutrient absorption alone.
Studies using this system address intestinal inflammation, host-microbe interactions, metabolic disease, and responses to diet or therapeutics. These applications bring together digestive, microbial, epithelial, barrier, and immune perspectives. As a result, the mouse gut serves not only as a setting for studying digestion but also as a model for interconnected gastrointestinal and metabolic questions.