Ex Vivo Skin
Ex Vivo Skin - Related Videos
07:31
In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin
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Cited by 13 •
2014
The protocol for dynamic longitudinal imaging and selective laser lesion of nerve endings in reporter transgenic mice is...
07:56
A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
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Cited by 13 •
2014
Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.
03:02
Chemical-induced Two-stage Skin Carcinogenesis Model: An Experimental In Vivo Mouse Model of Skin Cancer
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2023
This video describes the procedure to induce two-stage skin carcinogenesis to study tumor initiation by topical application of chemicals. In the first step, a mutagen, DMBA, is applied onto the skin of a mouse model which initiates tumor formation by causing mutations in the stem cells, followed by the application of a growth stimulator, TPA, which accelerates skin papilloma formation.
08:29
Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
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Cited by 11 •
2014
We describe the dissection and ex vivo culture of mouse embryonic skin. The culture system maintains an air-liquid interface across the tissue surface and allows imaging on an inverted microscope. Melanoblasts, a component of the developing skin, are fluorescently labeled allowing their behavior to be observed using confocal microscopy.
10:31
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
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Cited by 16 •
2016
We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.
FAQs
Maintaining suitable culture media, temperature, hydration, and gas exchange helps excised skin remain viable outside the body. These conditions support continued activity in epidermal and dermal cells while preserving tissue architecture and local interactions. Because each factor contributes to tissue maintenance, controlled conditions are essential for obtaining measurements that reflect biological responses rather than deterioration after excision.
The retained organization of epidermal and dermal tissue allows researchers to examine responses within a structured biological environment. Cells remain associated with neighboring cells and local tissue features, which can influence barrier behavior, wound healing, penetration, irritation, and toxicity measurements. This makes the model more biologically representative for these questions than experiments that examine isolated cells alone.
Ex vivo skin occupies an intermediate position between simplified cell cultures and whole-animal studies. It preserves tissue architecture and local interactions that cell cultures may lack, while allowing controlled laboratory measurements without studying the response of an entire animal. Its limited period of viability also means that it complements, rather than replaces, other experimental models.
Results depend strongly on how the tissue is maintained after excision. The culture medium, temperature, hydration, and gas exchange all contribute to continued viability and can influence measurements of barrier function or treatment responses. Researchers therefore need controlled conditions when comparing samples, because changes in tissue maintenance may be confused with effects caused by the experimental treatment.
A typical workflow begins with excision of skin samples, followed by placement in suitable culture conditions. The tissue is maintained with appropriate media, temperature, hydration, and gas exchange so that it remains viable for a limited period. Researchers can then apply the planned experimental assessment and measure responses related to architecture, barrier behavior, healing, penetration, irritation, or toxicity.
This model is useful when researchers need to observe biological responses in intact skin tissue under controlled laboratory conditions. Preserved epidermal and dermal organization supports investigations of wound healing and barrier function while allowing experimental measurements over a limited period. The approach can therefore connect tissue-level behavior with defined laboratory conditions more effectively than isolated-cell testing.
Ex vivo skin provides a tissue-based setting for investigating drug and cosmetic penetration, as well as irritation and toxicity. Researchers can maintain the sample under controlled conditions and assess how the tissue responds to the tested substance. These studies provide biologically relevant information while complementing cell cultures and animal studies in dermatological and biological research.