Oxygen Controlled Workstation
Oxygen Controlled Workstation - Related Videos
11:49
Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
0 Views •
Cited by 10 •
2013
Microfluidic oxygen control confers more than just convenience and speed over hypoxic chambers for biological experiments. Especially when implemented via diffusion through a membrane, microfluidic oxygen can provide simultaneous liquid and gas phase modulations at the microscale-level. This technique enables dynamic multi-parametric experiments critical for studying islet pathophysiology.
06:44
Tuning Oxide Properties by Oxygen Vacancy Control During Growth and Annealing
0 Views •
Cited by 1 •
2023
Oxide materials show many exotic properties that can be controlled by tuning the oxygen content. Here, we demonstrate the tuning of oxygen content in oxides by varying the pulsed laser deposition parameters and by performing postannealing. As an example, electronic properties of SrTiO3-based heterostructures are tuned by growth modifications and annealing.
08:23
Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
0 Views •
Cited by 16 •
2019
Here, we present oxygen gradient ektacytometry, a rapid and reproducible method to measure red blood cell deformability in samples from patients with sickle cell disease under controlled deoxygenation and reoxygenation. This technique provides a way to study red blood cell sickling and to monitor sickle cell disease treatment efficacy.
07:12
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
0 Views •
Cited by 26 •
2020
Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
0 Views •
Cited by 27 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
FAQs
The enclosure provides a contained work area, while oxygen sensors measure the internal atmosphere. Controlled gas exchange then changes the enclosed environment when measurements differ from the selected target. This coordinated arrangement allows experiments to be performed under oxygen-rich, atmospheric, or hypoxic conditions rather than relying on uncontrolled room conditions.
Sensors provide the measurement needed to determine whether the internal oxygen level matches the experimental target, while gas exchange provides the means to alter that level. Treating these as separate but coordinated functions explains how the system can stabilize conditions and why both measurement and atmospheric adjustment matter for reproducible biological experiments.
Oxygen availability can influence metabolism, signaling, growth, and survival. Holding a chosen concentration steady helps distinguish effects associated with oxygen conditions from effects caused by changing atmospheric oxygen during the experiment. This is particularly important when comparing oxygen-rich, atmospheric, and hypoxic settings across biological samples or experimental groups.
A typical use begins by placing biological samples or experimental materials within the enclosed work area, selecting the desired oxygen condition, and allowing the sensors and gas-exchange system to regulate the atmosphere. Researchers can then handle samples or conduct the experiment under the chosen setting, reducing exposure to uncontrolled oxygen fluctuations during the work.
This approach is useful when oxygen availability is itself an experimental variable, including cell culture, tissue studies, and organ studies. It enables investigators to examine how different oxygen settings affect biological responses rather than treating oxygen as a fixed background condition. The controlled environment can support comparisons among models and experimental conditions.
Experiments conducted under controlled oxygen conditions can be interpreted alongside outcomes such as changes in metabolism, signaling, growth, or survival. The workstation establishes the oxygen context in which these biological responses occur rather than replacing their measurement. More stable conditions can improve reproducibility by limiting atmospheric variation as a source of differing results.