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Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
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Bioengineering
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JoVE Journal Bioengineering
Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level

Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level

DOI:
10.3791/50616-v

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11:49 min

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November 17, 2013

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, , , , , ,
1Department of Mechanical Engineering,University of Michigan-Dearborn, 2Department of Surgery/Transplant,University of Illinois at Chicago, 3Department of Bioengineering,University of Illinois at Chicago

Chapters

  • 00:05Title
  • 01:59Microfluidic Platform Fabrication
  • 05:17Microdispenser Setup
  • 06:53Isolation and Staining of Mouse Islets
  • 08:25Simultaneous Oxygen and Glucose Stimulations
  • 09:19Results: Intermittent Hypoxia Preconditioning Improves Islet Responses to Hypoxia
  • 10:46Conclusion

Summary

Automatic Translation

Automatic Translation

Microfluidic oxygen control confers more than just convenience and speed over hypoxic chambers for biological experiments. Especially when implemented via diffusion through a membrane, microfluidic oxygen can provide simultaneous liquid and gas phase modulations at the microscale-level. This technique enables dynamic multi-parametric experiments critical for studying islet pathophysiology.

Tags

Keywords: Oxygen MicroenvironmentIsletMicrofluidicHypoxiaGlucose Stimulus-secretion CouplingCalcium DynamicsKATP ChannelPreconditioningReal-time Monitoring

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