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A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

DOI:
10.3791/59842-v

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07:12 min

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April 24, 2020

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, , , , ,
1Advanced Clinical Biosystems Institute, Smidt Heart institute,Cedars Sinai Medical Center, 2Beckman Coulter Life Sciences

Chapters

  • 00:04Introduction
  • 01:11Operating Procedure
  • 03:28Liquid Chromatography-Tandem Mass Spectrometry (LC-MSMS)
  • 04:38Results: Representative Mass Spectrometric Protein Sample Analysis
  • 06:32Conclusion

Summary

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Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.

Tags

Keywords: Automated WorkflowProtein Enzymatic DigestionReproducibilityThroughputBiomarker DiscoveryValidationClinical ApplicationMass SpectrometryHigh Throughput Sample PreparationQuantitative Proteomic AnalysisPlasma SampleLiquid HandlerAutomated Proteomic Sample PreparationHigh Content

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