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JoVE Journal
Biochemistry

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A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
 

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Article doi: 10.3791/59842
April 24th, 2020

Summary April 24th, 2020

Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.

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