Pipette Tips
Pipette Tips - Related Videos
05:22
Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
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Cited by 1 •
2022
This protocol allows for the preparation of transverse sections of cereal seeds (e.g., rice) for the analysis of endosperm and starch granule morphology using scanning electron microscopy.
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
06:35
Introduction to Serological Pipettes and Pipettors
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2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
08:11
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
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Cited by 7 •
2017
Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.
FAQs
A secure seal allows piston movement to change the air pressure inside the tip as intended. If the attachment is not properly seated, the pressure change may not produce the selected liquid movement accurately. Checking the fit before aspiration supports reliable volume transfer, which is especially important when preparing reagents or performing measurements in biological assays.
Immersion depth and plunger movement influence how consistently liquid enters and leaves the tip. Keeping the tip at an appropriate depth and operating the plunger smoothly helps support precise aspiration and dispensing. These technique factors become important during serial dilutions, where small transfer inconsistencies can affect the composition of subsequent samples.
Standard tips provide general-purpose liquid handling, while low-retention tips are selected for sample types in which reducing liquid remaining in the tip is important. Filter tips are used when contamination control is a priority. Choosing among these designs connects the tip to the sample and experimental requirement rather than treating all tips as interchangeable.
Selection should reflect the handling task, the sample, and the need for contamination control. Researchers may choose standard tips for routine transfers, low-retention tips for particular sample-handling needs, or filter tips when reducing contamination risk is important. Matching the tip design to the workflow helps support accurate, reproducible preparation of reagents and assay samples.
Tips enable measured transfers between reagents, cultures, and dilution steps. During serial dilution, the pipette moves a selected volume from one sample into another, so consistent aspiration and dispensing help preserve the intended sequence of concentrations. In reagent preparation, appropriate tip selection and controlled handling support reliable volumes across the workflow.
Sample loading requires transferring measured liquid into the assay system without compromising volume consistency or introducing avoidable contamination. A suitable tip and controlled pipetting technique help maintain reproducibility across samples. This matters because differences in transferred volume or contamination status can influence how biological assay results compare between experimental conditions.