Pressure Perfusion Fixation
Pressure Perfusion Fixation - Related Videos
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
09:38
Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
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Cited by 18 •
2015
The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.
04:19
Pressure Fixation of Isolated Murine Pulmonary Valve: A Procedure to Isolate and Chemically Fix the Mouse Pulmonary Valve in Closed Conformation
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2025
In this video, we describe the procedure to isolate and chemically fix the mouse pulmonary value in closed conformation via perfusion pressurization.
07:19
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
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Cited by 3 •
2021
Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.
05:48
Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice
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Cited by 4 •
2019
Presented here is a useful protocol for lung fixation that creates a stable condition for histological evaluate of lung specimens from a mouse model of emphysema. The main advantage of this model is that it can fix many lungs with the same constant pressure without lung collapse or deflation.
FAQs
Controlled fluid pressure creates the driving force that carries fixative through vessels and into capillary networks. As the fixative advances, it replaces blood or other fluids within the vascular system, allowing stabilization to begin throughout the organ rather than only at exposed surfaces. This vascular delivery helps preserve cellular and structural details across complex tissues.
Pressure must be controlled because the distribution of fixative depends on the conditions used to drive fluid through the vasculature. Consistent pressure, together with consistent fixation conditions, supports more uniform tissue preservation and improves reproducibility between specimens. These factors help maintain tissue integrity for later dissection, histology, immunohistochemistry, or microscopy.
Paraformaldehyde can serve as the fixative introduced through the vascular system. Once distributed through the tissue, it rapidly stabilizes proteins, helping preserve cellular organization and structural details. This stabilization also limits enzymatic degradation, which is important when organs must remain intact for subsequent biological analysis rather than being examined immediately after collection.
Complex, highly vascularized organs benefit particularly from this approach because their internal architecture can be reached through the vasculature. The brain is a prominent example, as pressure-driven distribution supports preservation throughout its intricate tissue structure. More broadly, the method is suited to specimens in which maintaining organ-wide cellular and structural relationships is important.
The process begins by introducing a suitable fixative through the specimen’s vasculature under controlled pressure. The fluid is driven through the vascular and capillary networks while replacing blood or other fluids, allowing fixation to proceed throughout the organ. After preservation, the specimen can undergo dissection, histology, immunohistochemistry, or microscopy.
Researchers would choose pressure perfusion fixation when they need to preserve the architecture of an intact, highly vascularized organ before detailed analysis. Its value is especially clear for brain studies and other complex tissues where internal structure matters. Preserved specimens can support tissue sectioning, staining-based examination, immunohistochemistry, and microscopic assessment.