Rfp-ara7
Rfp-ara7 - Related Videos
06:07
A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila
0 Views •
Cited by 1 •
2009
We describe a novel red fluorescent protein (RFP) reporter that is expressed specifically in the Drosophila eye. We detail a methodology for dissection of the eye imaginal disc and how this reporter can be used to aid in the dissection and identification of specific cell types in the developing eye.
06:49
Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
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2022
Using the low-cost cationic polymer polyethylenimine (PEI), we produced lentiviral particles for stable expression of shRNAs in H9 human embryonic stem cells (hESCs) and transiently transduced H9-derived neural progenitor cells (NPCs) at high efficiency.
04:21
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
0 Views •
Cited by 3 •
2024
The presented method describes the generation of a CRISPR-mediated gene knockout in the human embryonic stem cell (hESC) line H9, which stably expresses sgRNAs targeting the L2HGDH gene using a highly efficient lentiviral-mediated gene delivery system.
08:39
Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
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Cited by 8 •
2015
In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.
13:45
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
0 Views •
Cited by 7 •
2010
This paper describes the methodology to determine the chemotactic response of leukocytes to specific ligands and identify interactions between the cell surface receptors and cytosolic proteins using live cell imaging techniques.
FAQs
The red fluorescent tag remains associated with ARA7, so its signal changes position as the GTPase associates with endosomal membranes. This links fluorescence patterns to the distribution of ARA7 within the cell rather than merely marking membrane structures independently. Researchers can therefore use the reporter to follow where ARA7-positive compartments occur during endosomal trafficking.
ARA7-associated fluorescence identifies compartments connected with the route toward the vacuole, including pre-vacuolar compartments and multivesicular bodies. These structures are important locations for membrane transport and protein sorting. Observing their distribution helps researchers examine how cargo-bearing membrane compartments are organized before delivery to the vacuole in plant cells.
Imaging RFP-ARA7 can reveal changes in the formation, movement, and maturation of endosomal compartments. Comparing fluorescent structures over time provides a way to examine how vesicles and related compartments participate in membrane transport. This dynamic view adds information about trafficking behavior that cannot be obtained from compartment location alone.
RFP-ARA7 provides a visible reference for endosomal compartments involved in sorting and vacuole-directed transport. Researchers can relate the presence and behavior of these ARA7-associated structures to the organization of pathways that direct cellular cargo. In this way, the reporter supports analysis of how membrane trafficking contributes to plant cell organization.
Researchers examine the fluorescent distribution of ARA7-associated endosomal membranes within living plant cells. The resulting patterns can identify pre-vacuolar compartments and multivesicular bodies while preserving information about their movements and changing organization. This live-cell context is useful for connecting compartment appearance with ongoing membrane transport rather than viewing trafficking as a static arrangement.
The reporter is especially useful when a study focuses on endosomal trafficking, membrane transport, protein sorting, or delivery toward the vacuole in plants. It can help researchers investigate how vesicles and endosomal compartments are organized and how they behave during trafficking. Its value comes from linking visible fluorescence with ARA7-associated transport pathways in living cells.