Rna Containing Oligos
Rna Containing Oligos - Related Videos
11:44
Synthesis and Mass Spectrometry Analysis of Oligo-peptoids
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Cited by 6 •
2018
A protocol is described for the manual synthesis of oligo-peptoids followed by sequence analysis by mass spectrometry.
08:43
OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
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Cited by 35 •
2010
A rapid way is described to gain insights into the structure of polysaccharides in an extracellular matrix. The method takes advantage of the specificity of glycosylhydrolases and the sensitivity of mass spectrometry allowing minute amounts of materials to be analyzed. This technique is adaptable to be used directly on tissue itself.
16:42
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
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Cited by 4 •
2010
This work shows how to form an RNA/DNA hybrid at the chromosomal level and reveal transfer of genetic information from RNA to genomic DNA in yeast cells.
09:54
RNA-Seq
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2023
Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed and analyzed without relying on prior available genomic information. During RNA-seq, RNA isolated from samples of interest is used to generate a DNA library, which is then amplified and sequenced. Ultimately, RNA-seq can determine which genes are expressed, the levels of their expression, and the presence of any previously unknown transcripts.
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Cited by 20 •
2017
Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.
FAQs
Complementary base pairing allows an oligo to recognize a matching sequence in RNA or DNA. The extent to which its sequence corresponds to a target determines which nucleic acid region it can interact with, making sequence recognition central to probes, primers, and antisense reagents. This specificity enables researchers to examine particular nucleic acid molecules rather than bulk cellular material.
The ribose 2′-hydroxyl group influences strand conformation, hybridization behavior, and susceptibility to degradation. These properties affect how an RNA-containing oligo interacts with a complementary RNA or DNA strand and how stable it remains during an experiment. Consequently, the ribonucleotide content is relevant when interpreting recognition, binding, and persistence in nucleic acid studies.
Their sequence-specific interactions provide a common basis for several functions, while the experimental role determines how researchers apply them. A sequence can serve as a molecular probe for detection, a primer for nucleic acid analysis, or an antisense reagent in gene-regulation studies. These uses connect the same recognition principle to measurement, analysis, and functional investigation.
Hybridization behavior describes how readily an oligo forms a paired structure with a complementary RNA or DNA sequence. Because the ribose 2′-hydroxyl group influences this behavior, it can affect the interaction observed in an experiment. Researchers therefore consider hybridization when using these strands to detect nucleic acids or examine sequence-specific molecular interactions.
As molecular probes, these oligos use complementary base pairing to identify a matching RNA or DNA sequence. The resulting sequence-specific interaction supports detection and analysis of nucleic acids, allowing researchers to investigate whether a particular target can be recognized. This application is especially relevant when experiments focus on examining nucleic acid identity or distribution through targeted molecular interactions.
Antisense reagents use sequence-specific recognition to participate in studies of gene regulation. By targeting a complementary nucleic acid sequence, they provide an experimental approach for examining RNA function and investigating mechanisms associated with gene expression control. Their value lies in enabling researchers to connect a defined nucleic acid interaction with broader questions about regulation and cellular information flow.