Sarcoplasm

Sarcoplasm is the specialized cytoplasm of a muscle fiber, where the structures and molecules that support contraction, energy production, and cellular maintenance are organized. It contains myofibrils, mitochondria, glycogen, myoglobin, and the sarcoplasmic reticulum; when a muscle fiber is stimulated, the sarcoplasmic reticulum releases calcium ions into the sarcoplasm, enabling interactions between actin and myosin, while mitochondria supply ATP for cross-bridge cycling. Studying sarcoplasm helps explain how muscle cells generate force, manage oxygen and fuel reserves, and adapt to exercise, disease, or injury. Its organization is central to research in physiology, histology, sports science, and muscle-related disorders.

Sarcoplasm - Related Videos

Research

JoVE Journal - Biology

Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

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Cited by 8 •

2015

This article describes the detailed protocol and equipment necessary for dual optical mapping of transmembrane potential (Vm) and free intra-sarcoplasmic reticulum (SR) Ca2+ in the Langendorff-perfused rabbit heart. This method allows for direct observation and quantification of Vm and SR Ca2+ dynamics in the intact heart.

Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

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Cited by 18 •

2017

Intracellular calcium recycling plays a critical role in regulation of systolic and diastolic function in cardiomyocytes. Here, we describe a protocol to evaluate sarcoplasmic reticulum Ca2+ reserve and diastolic calcium removal function in cardiomyocytes by a calcium imaging system.

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

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Cited by 1 •

2016

Currently, most available calcium indicators are used to quantify cytoplasmic calcium transients as indirect measures of calcium released from the sarcoplasmic reticulum in cultured smooth muscle cells. This protocol describes the use of a specific FRET-based indicator that allows direct measurement of calcium signals within the sarcoplasmic reticulum lumen.

Research

JoVE Journal - Developmental Biology
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In Vitro Differentiation of Mature Myofibers for Live Imaging

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Cited by 38 •

2017

Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.

Research

JoVE Journal - Biology
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Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

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Cited by 29 •

2013

We describe the isolation of human atrial myocytes which can be used for intracellular Ca2+ measurements in combination with electrophysiological patch-clamp studies.

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