Surface Micro-extraction
Surface Micro-extraction - Related Videos
07:16
Extraction of the EPP Component from the Surface EMG
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Cited by 2 •
2009
The endplate potential (EPP) component can be extracted from the surface EMG using a digital filter. The extracted EPP shows oscillation with a frequency of about 30 Hz.
07:42
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
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Cited by 7 •
2010
This video shows experiments with subsequent analysis of protein-protein interactions by the use of micro-patterned surfaces. The approach offers the possibility to detect protein interactions in living cells and combines high throughput capabilities with the possibility to extract quantitative information.
02:59
Extraction of Viral RNA from Surface-Bound Virions Using a Silica Spin Column
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2026
Source: Klaus, J. P. et al. Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment. J. Vis. Exp. (2016)This video demonstrates the extraction of viral RNA from surface-bound virions on epithelial cells using silica spin columns. The method includes chemical lysis, RNA stabilization, and membrane-based purification for downstream quantification of virus-cell attachment.
05:31
Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region
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Cited by 170 •
2007
Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.
09:37
Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data
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Cited by 4 •
2016
A methodology for obtaining visual and quantitative root structure information from X-ray computed tomography data acquired in-soil is presented.
FAQs
When the coated probe, sorptive material, or small solvent phase contacts a target surface, chemical compounds partition from that surface into the extracting phase. The extracted material is then desorbed or transferred for instrumental measurement. This sequence concentrates surface-associated molecules before analysis, allowing a small sampled region to provide chemically informative material.
The probe or sorptive material provides the phase that receives compounds from the contacted surface. Its small format supports localized sampling while limiting the amount of sample and solvent required. After contact, the retained analytes can be desorbed or transferred to an instrument, linking the physical sampling step with chemical measurement.
Localized contact lets investigators examine chemical composition at a defined position rather than treating an entire specimen as uniform. In biological analysis, this supports spatially resolved measurements across tissues, cells, biomaterials, or laboratory surfaces. The approach can therefore retain positional information while using limited material from specimens that may be complex or difficult to replace.
A typical workflow begins by bringing a coated probe, sorptive material, or small solvent phase into contact with the selected surface. Compounds move into the extracting phase during contact. The collected material is then desorbed or transferred for instrumental measurement, producing an analytical record of chemical residues present at the sampled location.
In biology, Surface micro-extraction can be applied to tissues, cells, biomaterials, and laboratory surfaces. The collected compounds may include metabolites, lipids, drugs, and other molecular residues. This range makes the technique useful when researchers need chemical information from a particular biological or experimental surface rather than from a large, homogenized sample.
The method is especially useful when specimens are limited, complex, or require localized examination. Its low sample consumption can help preserve biological material, while direct surface sampling supports spatially resolved analysis. Researchers can consequently investigate molecular residues on specific regions of tissues, cells, biomaterials, or laboratory surfaces without requiring extensive sample preparation described in the source.