Vascular Regeneration
Vascular Regeneration - Related Videos
07:32
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
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Cited by 7 •
2014
The rodent retina has long been recognized as an accessible window to the brain. In this technical paper we provide a protocol that employs the mouse model of oxygen-induced retinopathy to study the mechanisms that lead to failure of vascular regeneration within the central nervous system after ischemic injury. The described system can also be harnessed to explore strategies to promote regrowth of functional blood vessels within the retina and CNS.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
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Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
09:23
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
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Cited by 40 •
2015
In this work, we present a technique for the rapid fabrication of living vascular tissues by direct culturing of collagen, smooth muscle cells and endothelial cells. In addition, a new protocol for the mechanical characterization of engineered vascular tissues is described.
09:14
An Introduction to Aging and Regeneration
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2023
Tissues are maintained through a balance of cellular aging and regeneration. Aging refers to the gradual loss of cellular function, and regeneration is the repair of damaged tissue generally mediated by preexisting adult or somatic stem cells. Scientists are interested in understanding the biological mechanisms behind these two complex processes. By doing so, researchers may be able to use somatic stem cells to treat degenerative diseases and develop therapies that could delay the effects of...
06:56
A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
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Cited by 7 •
2011
In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.
FAQs
Angiogenic signals provide cues that stimulate the vascular response, while the extracellular matrix supplies a surrounding framework for endothelial-cell interactions. Cells in the local tissue also influence how this response develops. Because vascular regeneration depends on these signals, matrix relationships, and neighboring-cell interactions together, studying them helps explain why new vessel structures form in some repair settings and supports regenerative-medicine design.
Endothelial-cell proliferation increases the number of cells available for repair, migration places those cells where new vascular structures are needed, and organization arranges them into vessel structures. These activities are interdependent rather than isolated events. Examining their coordination gives researchers a way to evaluate whether a regenerative response can reestablish tissue perfusion and oxygen delivery rather than merely produce additional endothelial cells.
Ischemic tissues require restoration of blood supply to improve delivery of oxygen. Vascular regeneration is therefore studied as a route to reestablish perfusion after injury or disease. Its biological significance lies in connecting endothelial-cell activity and new vessel formation with tissue recovery. This focus makes ischemic-tissue models useful for examining whether regenerative strategies improve the blood supply available to damaged tissue.
Researchers examine endothelial-cell proliferation, migration, and organization, together with angiogenic signals, extracellular-matrix interactions, and surrounding-cell effects. They then relate these biological features to the restoration of tissue perfusion and oxygen delivery. This framework can be used in models of tissue recovery, allowing investigators to connect cellular behavior with the larger outcome of reestablishing a blood supply.
It is relevant whenever tissue recovery depends on restoring a blood supply. In wound-healing studies, researchers investigate how vascular responses accompany repair. In cardiovascular research, the same biological principles can guide therapies for disease-related loss of perfusion. The topic therefore links basic biology of endothelial cells and vessel formation with efforts to understand or improve tissue recovery.
Engineered grafts and vascularized tissues must be considered in relation to blood-vessel formation and tissue perfusion. Studying the cellular and signaling requirements of vascular regeneration provides biological context for developing these constructs. Researchers can use this knowledge to assess whether an engineered tissue supports the formation or restoration of vessels needed for oxygen delivery, an important consideration in regenerative medicine.