Whole Cell Recording
Whole Cell Recording - Related Videos
09:27
Whole Cell Recordings from Brain of Adult Drosophila
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Cited by 39 •
2007
This video demonstrates the procedure for isolating whole brains from adult Drosophila in preparation for recording from single neurons using standard whole cell technology. It includes images of GFP labeled cells and neurons viewed during recording.
11:52
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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Cited by 1 •
2017
We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.
07:23
Whole-cell Patch-clamp Recordings in Brain Slices
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Cited by 57 •
2016
This protocol describes basic procedural steps for performing whole-cell patch-clamp recordings. This technique allows the study of the electrical behavior of neurons, and when performed in brain slices, allows the assessment of various neuronal functions from neurons that are still integrated in relatively well preserved brain circuits.
13:07
One-channel Cell-attached Patch-clamp Recording
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Cited by 17 •
2014
Described here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp technique. This method allows for observing, in real time, the pattern of open-close channel conformations that underlie the biological signal. These data inform about channel properties in undisturbed biological membranes.
03:56
Whole-Cell Voltage Clamp Recordings from Drosophila Photoreceptors
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2025
The video demonstrates recording electrical activity in the photoreceptor neurons of Drosophila's ommatidium by establishing a whole-cell configuration. It highlights the activation of transient receptor potential channels by light pulses, resulting in measurable quantum bumps.
FAQs
The seal and membrane rupture perform different jobs. The tight seal connects the glass micropipette closely to the cell membrane, while gentle suction ruptures the membrane directly beneath it. This sequence provides access to the cell interior, allowing investigators to control or measure membrane voltage and ionic currents during the recording.
Whole-cell recording can be used in two complementary ways: researchers may control membrane voltage and observe the resulting ionic currents, or measure voltage changes produced by the cell. This distinction matters because voltage and current provide different views of excitability, including rapid electrical events and responses associated with communication between cells.
Different readouts answer different biological questions. Action potentials reveal electrical signaling events, synaptic responses indicate how communication affects the recorded cell, ion-channel activity provides information about membrane function, and changes in membrane properties show altered cellular electrical behavior. Together, these outcomes connect membrane-level measurements with the physiology of excitable cells.
The researcher positions a glass micropipette against the cell membrane, forms a tight seal, and applies gentle suction to rupture the membrane beneath the pipette. After electrical access to the cell interior is established, membrane voltage or ionic currents can be directly controlled or measured during recording.
The glass micropipette is central because it both forms the membrane seal and provides the route for electrical access after membrane rupture. Its role links the physical preparation of the cell to the measurement itself. Consequently, this component is essential for obtaining voltage or current recordings.
Researchers choose Whole Cell Recording when they need electrical measurements from an entire cell in contexts such as cellular physiology, neural signaling, pharmacology, or disease mechanisms. In biology, the approach can be applied to neurons, muscle cells, and other excitable cells to examine communication and changes in membrane behavior.