Proteus Mirabilis
Proteus Mirabilis - Related Videos
07:10
Super-resolution Imaging of Proteus mirabilis Biofilm by Expansion Microscopy
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2025
This article presents a comprehensive protocol for PmbExM, an Expansion Microscopy technique designed specifically for Proteus mirabilis biofilms. PmbExM utilizes a stepwise enzymatic treatment of biofilm samples to achieve an isotropic, 4.3-fold expansion, enabling super-resolution analysis of the spatial organization of cellular and subcellular structures within these sessile microbial communities.
06:51
A Minimally Invasive Model of Aortic Stenosis in Swine
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Cited by 1 •
2023
This protocol describes a minimally invasive surgical procedure for ascending aortic banding in swine.
12:07
Physiological Preparation of Hair Cells from the Sacculus of the American Bullfrog (Rana catesbeiana)
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Cited by 8 •
2017
The American bullfrog's (Rana catesbeiana) sacculus permits direct examination of hair-cell physiology. Here the dissection and preparation of the bullfrog's sacculus for biophysical studies is described. We show representative experiments from these hair cells, including the calculation of a bundle's force-displacement relation and measurement of its unforced motion.
09:24
Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
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Cited by 10 •
2017
This technique describes an efficient screening process for evaluating bacteria-specific optical imaging agents within ex vivo human lung tissue, by fibered confocal fluorescence microscopy for the rapid identification of small molecule chemical probe-candidates with translatable potential.
05:29
Axoplasm Isolation from Rat Sciatic Nerve
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Cited by 6 •
2010
We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.
FAQs
Swarming motility allows Proteus mirabilis to move across surfaces rather than remaining confined to a single location. This behavior is clinically relevant because surface movement can support spread along environments associated with urinary devices and may contribute to persistent colonization. Studying motility therefore helps explain how the organism establishes and maintains catheter-associated infection.
Urease changes the urinary environment by hydrolyzing urea, which raises urine pH. The resulting alkalinity favors precipitation of struvite and carbonate apatite crystals. These deposits can contribute to infection-associated kidney or bladder stones, making urease activity important not only for understanding disease mechanisms but also for interpreting why infection may become persistent.
Biofilm formation provides a surface-associated context for studying persistence in catheter-associated disease. In combination with swarming motility and urease activity, it helps explain why colonization may continue in the urinary tract rather than resolving quickly. Investigating biofilms can connect bacterial behavior with device-associated infection and clarify mechanisms not captured by examining motility or urease alone.
Laboratory identification combines culture with biochemical testing to support a clinical diagnosis. These results help distinguish the causative organism within the clinical investigation and inform antimicrobial selection. Because identification also contributes to infection-control decisions, the workflow links microbiological findings with treatment choices and control of device-associated disease.
Long-term urinary catheters and urinary tract abnormalities create clinical settings in which persistent colonization is especially important. In these patients, surface movement, urease activity, and biofilm formation can be considered together rather than as isolated traits. This integrated view helps relate laboratory observations to catheter-associated disease and infection-associated stone formation.
A clinical investigation can combine culture and biochemical testing with examination of swarming motility, urease activity, and biofilm formation. The first approaches support diagnosis and antimicrobial selection, while the behavioral studies clarify mechanisms of catheter-associated disease and persistent colonization. Together, these findings also provide information relevant to infection control and infection-associated stone formation.