Axoplasm Isolation from Rat Sciatic Nerve

Journal

Neuroscience

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Axoplasm Isolation from Rat Sciatic Nerve

DOI:

10.3791/2087-v

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05:29 min

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September 24, 2010

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Ida Rishal, Meir Rozenbaum, Mike Fainzilber

¹Department of Biological Chemistry,Weizmann Institute of Science

Chapters

00:00Title
00:50Introduction
01:38Dissection of Sciatic Nerves
03:16Incubation, Washing, and Elution of Axoplasm
04:41Evaluation of Axoplasm Purity
05:06Conclusion

Summary

Automatic Translation

We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.

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