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JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Axoplasm Isolation from Rat Sciatic Nerve
 

Axoplasm Isolation from Rat Sciatic Nerve

Article doi: 10.3791/2087
September 24th, 2010 • Usage Statistics
1, 1, 1
1Department of Biological Chemistry, Weizmann Institute of Science

Summary
Automatic Translation
September 24th, 2010

We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.

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