Ifn-gamma Secretion Detection
Ifn-gamma Secretion Detection - Related Videos
11:53
ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes
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2023
Source: Tonya J. Webb1 1 Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center, Baltimore, Maryland 21201 ELISPOT is a standardized, reproducible assay used to detect cellular immune responses. The assay utilizes an enzyme-linked immunosorbent assay (ELISA)- based method to detect single-cell immune responses which can be visualized by spots, hence the name ELISPOT. ELISPOT was first described in...
05:28
Assay for the Identification of Interferon-Gamma Secretion in Murine Lymphoid Organs
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2025
This video demonstrates an imaging method to visualize in situ interferon-gamma (IFN-γ) secretion in the mouse spleen. Following bacterial infection and the induction of IFN-γ accumulation inside T cells and natural killer cells, the spleen is harvested from the mouse. Upon obtaining tissue sections, immunostaining is performed to visualize IFN-γ inside the cells.
08:04
Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
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Cited by 9 •
2014
Novel generations of functional assays such as gamma interferon (IFN-γ) ELISpot, which detect cytokine production at the single cell level and provide both quantitative and qualitative characterization of T cell responses can be used to assess cell-mediated immune responses directed against varicella zoster virus (VZV).
01:32
Evoked Gamma Oscillations for the Treatment of Alzheimer’s Disease
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2025
Begin with a patient diagnosed with Alzheimer’s disease, wearing a gamma therapy stimulation device.The device consists of an eye-set and headphones.Using the controller, activate the device to deliver auditory and visual stimulation.The visual component emits flickering light at a gamma frequency, while the auditory component produces synchronized sound pulses.Gamma oscillations are brain waves generated by synchronized neuronal firing, crucial for cognition and memory.In AD, these...
05:00
Localized Surface Plasmon Resonance Imaging to Detect Protein Secretions from a Single Cell
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2025
This video demonstrates localized surface plasmon resonance imaging to detect protein secretions from a single cell. Using a microfluidic setup and a peptide-bound plasmonic nanostructure chip, changes in reflected light intensity at the resonance angle reveal interactions with secreted proteins from a single cell.
FAQs
The antibody step determines how released cytokine becomes measurable: specific antibodies capture or label IFN-γ after immune-cell activation. This links a molecular signal to a functional assay result rather than merely indicating that lymphocytes are present. Depending on the format, the measurement can emphasize overall secretion, responding-cell frequency, or antigen-specific activity.
Stimulation choice defines the immune response being examined. Antigen exposure supports analysis of antigen-specific activity, whereas a mitogen provides a different activation condition for comparison. Evaluating these conditions helps investigators determine whether measured IFN-γ reflects responses associated with a selected antigen or with broader immune-cell activation in the experimental system.
These formats emphasize different aspects of the response. ELISA measures cytokine in a sample, ELISpot reveals responding-cell frequencies through localized secretion signals, and intracellular cytokine staining identifies cytokine production within cells. Together, the methods can address secretion levels, the proportion of responding cells, or antigen-specific activity, depending on the experimental objective.
A typical workflow begins by exposing lymphocytes to an antigen or mitogen, followed by capturing or labeling the IFN-γ released by activated cells with specific antibodies. The selected assay format then quantifies the signal. Investigators interpret that measurement as evidence of functional immune activity and compare it across experimental conditions.
This approach is useful when researchers need to evaluate cell-mediated immune responses rather than cell presence alone. In infection and immunology studies, it supports investigation of host-pathogen interactions, immune competence, vaccine responses, and disease-associated inflammation. Results can help compare experimental conditions and assess whether immune activity is associated with protective responses.
The assay can provide complementary information about how many immune cells respond, how much IFN-γ they release, and whether activity is directed toward a tested antigen. These outcomes allow investigators to compare immune responses between conditions, characterize functional differences, and connect cytokine secretion patterns with infection, inflammation, or vaccine-related studies.