Immune Cell Depletion
Immune Cell Depletion - Related Videos
06:17
Depletion of Specific Cell Populations by Complement Depletion
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Cited by 15 •
2010
To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
07:55
Ground State Depletion Super-resolution Imaging in Mammalian Cells
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Cited by 16 •
2017
This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.
11:00
Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy
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Cited by 10 •
2018
We present a protocol for using STED microscopy to simultaneously image actin structures, microtubules, and microtubule plus-end binding proteins in B cells that have spread on coverslips coated with antibodies to the B-cell receptor, a model for the initial phase of immune synapse formation.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
FAQs
Antibody-mediated depletion can remove targeted cells through two distinct effector routes: complement activation or Fc-mediated killing. Antibodies first recognize cell-surface markers, then recruit one of these mechanisms to reduce the marked population. Because the strategy depends on marker expression and downstream immune effector activity, the resulting depletion reflects both targeting specificity and the killing pathway engaged.
Cell-surface marker selection determines which immune population is targeted, while the extent of depletion influences how confidently a function can be assigned to that population. Incomplete targeting may leave enough cells to retain activity, whereas excessive depletion can alter broader immune responses and increase infection susceptibility. Both outcomes can confound conclusions about pathogen control or tissue damage.
Drugs or irradiation may suppress selected immune compartments or produce broader immune suppression, whereas antibody strategies are directed through cell-surface markers and can engage complement or Fc-mediated killing. This distinction affects how specifically investigators can alter the immune response. The choice of approach therefore shapes whether results reflect one defined leukocyte population or a wider loss of immune function.
Investigators should relate the intended cell population and depletion approach to the outcome being measured, such as pathogen clearance, inflammation, or tissue damage. They must also consider whether targeting was incomplete or excessive, because either condition can distort functional interpretation. Careful control of the depletion model helps distinguish a cell's contribution from secondary effects caused by altered immune susceptibility.
Depletion models help determine whether particular immune populations contribute to responses relevant to vaccines or therapies. By reducing selected T cells, B cells, macrophages, or other leukocytes, investigators can assess how those compartments influence pathogen clearance, inflammation, or tissue injury. These comparisons provide functional context for evaluating whether an intervention depends on, changes, or protects specific immune responses.
In infection research, reducing a defined population allows investigators to examine whether that compartment controls pathogen clearance, contributes to inflammation, or promotes tissue damage. T cells, B cells, macrophages, and other leukocytes may therefore be evaluated in relation to different disease outcomes. Interpretation requires caution, because broad or excessive depletion can itself increase susceptibility to infection and complicate the result.