Mouse Dendritic Cells
Mouse Dendritic Cells - Related Videos
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
02:57
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
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2023
This video describes a protocol for the generation of dendritic cells--key antigen-presenting cells that activate T cells--from the bone marrow of an adult mouse.
09:25
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
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Cited by 68 •
2012
Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.
09:09
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
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Cited by 8 •
2016
Distinct dendritic cell subsets exist as rare populations in lymphoid organs, and therefore are challenging to isolate in sufficient numbers and purity for immunological experiments. Here we describe a high efficiency, high yield method for isolation of all of the currently known major subsets of mouse splenic dendritic cells.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
FAQs
Danger signals are detected through pattern-recognition receptors, which connect environmental or pathogen-associated cues to the cell’s antigen-presenting activity. This sensing helps determine how captured material is handled and how strongly T cells are engaged. In infection studies, examining this relationship helps researchers evaluate how innate recognition shapes subsequent adaptive immune responses.
Antigen processing prepares captured material for display on major histocompatibility complex molecules, allowing T cells to examine antigen-derived information. The resulting presentation provides a communication point between antigen capture and T-cell responses. Studying this step helps clarify how immune recognition is initiated and how antigen presentation may influence infection or disease progression.
The activation state of these cells and the cytokines in their surroundings can shift the outcome of T-cell stimulation. Rather than producing one uniform response, they may support T-cell priming, influence immune polarization, or contribute to tolerance. This context dependence is important when interpreting immune responses during infection, vaccination, or immunotherapy research.
Researchers can examine whether antigen presentation leads to T-cell priming, alters the direction of immune polarization, or supports tolerance. These outcomes provide different views of how the immune system responds to antigen and danger signals. Comparing them helps investigators connect cellular sensing and presentation with broader host responses to infection and disease.
In infection research, these cells provide a model for following how pathogens or tissue-derived antigens are captured, sensed, processed, and presented to T cells. Investigators can therefore connect innate detection with downstream adaptive responses. The model is useful for examining host responses and for considering how antigen presentation may affect disease progression.
Their ability to link antigen handling with T-cell responses makes them relevant for evaluating vaccines and immunotherapies. Studies can focus on whether an intervention changes antigen presentation, strengthens T-cell priming, redirects immune polarization, or favors tolerance. These readouts help researchers assess how an approach may shape protective or disease-related immune responses.