Recombinant Herpesvirus Vectors
Recombinant Herpesvirus Vectors - Related Videos
02:41
Clonal Isolation and PCR Screening of GFP-Excised Recombinant Herpesvirus Retaining the Target Gene
0 Views •
2026
Source: Tang, N., et al. Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing. J. Vis. Exp. (2019)This video demonstrates the clonal isolation and PCR-based screening of recombinant herpesvirus following site-specific excision of a GFP marker. Fluorescence-activated cell sorting is used to isolate non-fluorescent infected cells, and junction PCR confirms retention of the target gene in GFP-negative clones.
12:21
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
0 Views •
Cited by 13 •
2019
Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.
08:35
Production and Titering of Recombinant Adeno-associated Viral Vectors
0 Views •
Cited by 149 •
2011
Recombinant adeno-associated virus (rAAVs) vectors are becoming increasingly valuable for in vivo studies in animals. We describe how rAAVs can be produced in the laboratory and how these vectors can be titered to give an accurate reading of the number of infectious particles produced.
08:33
Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector
0 Views •
Cited by 12 •
2016
This manuscript describes how viral vector-mediated local gene delivery provides an attractive way to express transgenes in the central nervous system. The protocol outlines all crucial steps to perform a viral vector injection in the substantia nigra of the rat to develop a viral vector-based animal model for Parkinson's disease.
03:29
Intravenous Administration of Recombinant Adeno-Associated Virus Vectors in a Rat Model
0 Views •
2025
Source: Grames, M. S., et al. Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction. J. Vis. Exp. (2017)This video demonstrates the tail vein injection of recombinant adeno-associated virus (AAV) in rats. After injection into the lateral tail vein, the virus enters the bloodstream, circulates through the heart, and reaches the brain, where it enters neurons and facilitates target protein production for...
FAQs
Design depends on balancing delivery performance with biological control. Researchers insert a selected transgene or antigen into the viral genome, then alter viral genes associated with replication, persistence, or pathogenicity. These changes aim to preserve efficient entry into host cells while improving safety. The resulting vector can deliver genetic material without retaining every property of the original virus.
Long-term infection makes these vectors useful for questions that require sustained observation of host-pathogen interactions. Because the vector can establish persistence, investigators can examine immune responses and gene-delivery effects over an extended period rather than focusing only on initial cell entry. Persistence also becomes an important design consideration alongside replication and pathogenicity when viral genes are modified.
These vectors can deliver antigens or other selected genes that allow researchers to examine antigen presentation and the resulting immune response. Their use can address both innate and adaptive immunity, rather than restricting analysis to a single immune mechanism. This makes them valuable for investigating how engineered viral delivery influences immune activation in immunology and infection studies.
A design begins by selecting a transgene or antigen for insertion into the herpesvirus genome. Researchers then modify viral genes that regulate replication, persistence, or pathogenicity, while seeking to retain efficient host-cell entry. The engineered vector is subsequently used for gene delivery or immune studies, allowing its design features to be examined in the intended research context.
They are useful when a study requires delivery of an antigen or another selected gene into host cells. In vaccine research, the inserted antigen supports investigation of immune responses. In gene-delivery studies, the platform provides a way to introduce genetic material while taking advantage of efficient cell entry and the vector's capacity for a large genome.
Recombinant herpesvirus vectors also support antigen presentation studies, investigations of host-pathogen interactions, and targeted therapeutic development. Their large genome capacity permits insertion of selected genetic material, while their ability to establish long-term infection helps researchers examine persistent biological relationships. Together, these properties connect engineered viral delivery with broader questions in immunology, infection, and therapeutic design.