Flat Mount Choroid Quantification
Flat Mount Choroid Quantification - Related Videos
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Kidney Flat Mount Preparation: A Procedure to Dissect and Prepare a Flat Mount for Microscopic Analysis of Adult Zebrafish Kidney
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2023
This video demonstrates the dissection and preparation of a flat mount of the adult zebrafish kidney. The prepared flat mount can be visualized under the microscope for specimen analysis.
08:02
Dissection and Flat-mounting of the Threespine Stickleback Branchial Skeleton
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Cited by 18 •
2016
The branchial skeleton, including gill rakers, pharyngeal teeth, and branchial bones, serves as the primary site of food processing in most fish. Here we describe a protocol to dissect and flat-mount this internal skeleton in threespine sticklebacks. This method is also applicable to a variety of other fish species.
06:36
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
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Cited by 50 •
2014
The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.
00:00
Calcium Imaging In Electrically Stimulated Flat-Mounted Retinas
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Cited by 2 •
2023
Retinal prostheses have the ability to generate visual perceptions. To advance the development of new prostheses, ex vivo methods are needed to test devices before implantation. This article provides a comprehensive protocol for studying calcium activity in the retinal ganglion cell layer when subjected to electrical stimulation.
04:05
Establishing a Whole-Cell Patch Clamp for Electrophysiological Recording from a Flat-Mount Mouse Retina
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2025
This video demonstrates a whole-cell patch-clamp technique for electrophysiological recording from a flat-mount mouse retina. A thin glass pipette with an electrode is used to approach starburst amacrine cells (SACs) within the retina. After forming a seal, suction is applied to rupture the membrane and form the whole-cell patch-clamp configuration. Finally, the flow of ions through the SAC synaptic receptors is measured using the configuration.
FAQs
Radial incisions allow the choroid-sclera complex to spread into a flat preparation rather than remain folded or curved. This geometry makes the vascular network accessible for microscopy and supports area and pattern measurements across the mounted tissue. Consistent flattening is important because distortion could affect estimates of vessel area, density, branching, or lesion size.
Microscopy and image-analysis software convert the labeled preparation into measurable observations. The images show the vascular structures, while analysis identifies or calculates features such as vessel area, density, branching, and lesion size. This combination links visual labeling with quantitative outputs, allowing researchers to compare vascular patterns rather than relying only on descriptive inspection.
The available metrics provide different quantitative readouts of the vascular preparation. Vessel area, density, and branching describe network structure, whereas lesion size quantifies the extent of a defined lesion. Reporting more than one parameter can give a broader assessment of vascular change and help distinguish overall network measurements from localized lesion measurements.
A basic workflow begins by removing the retina from the choroid-sclera complex. Researchers then make radial incisions, flatten and mount the remaining tissue, and label it for visualization. Microscopy provides images of the preparation, and image-analysis software is used afterward to quantify vascular or lesion-related parameters.
Researchers use this approach to study ocular vascular biology and diseases involving abnormal choroidal neovascularization. The method is particularly relevant to investigations of age-related macular degeneration, where measurements of vascular structure or lesion size can support evaluation of disease-related changes and experimental responses.
Because the method produces quantitative measurements of vascular features and lesions, researchers can compare treatment effects with changes associated with disease progression. Vessel area, density, branching, or lesion size provide measurable outcomes for assessing how the choroidal vascular network differs between experimental conditions or disease states.