Endothelial Cell Permeability
Endothelial Cell Permeability - Related Videos
03:20
Labeling Neural Cell Surface Markers with Azidosugar while Co-culturing with Endothelial Cells
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2025
This video demonstrates a protocol for co-culturing mouse brain endothelial cells and primary cortical stem cells and labeling the cell surface glycoproteins using Ac4ManNAz (azidosugar per-O-acetylated N-azidoacetylmannosamine). This method allows for selective enrichment and identification of cell surface proteins in primary cells.
07:03
An in vivo Assay to Test Blood Vessel Permeability
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Cited by 335 •
2013
We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces.
21:08
Engineering Cell-permeable Protein
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Cited by 12 •
2009
Protein transduction enables the direct delivery of biologically active proteins into cells. In contrast to conventional methods such as DNA transfection or viral transduction this non-invasive paradigm allows highly efficient cellular manipulation in a titratable manner circumventing cellular toxicity and the risk of oncogenic transformation by permanent genetic modification.
06:26
Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
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Cited by 6 •
2020
The human blood-brain barrier selectively prevents penetration of hydrophilic molecules and pathogens into the brain. Several pathologies, including meningitis and postoperative delirium, are associated with an increased permeability of the blood-brain barrier. Here, we describe an endothelial cell culture model to test the barrier permeability by microbial traversal.
11:04
Isolation of Valvular Endothelial Cells
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Cited by 82 •
2010
We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.
FAQs
Selective exchange follows two principal routes: substances may cross the endothelial cell itself, or they may pass through the spaces between neighboring cells. Intercellular junctions influence the second route, while membrane transporters and vesicular transport affect movement through cells. The relative contribution of these pathways helps determine which water, ions, solutes, or cells can reach surrounding tissue.
Intercellular junctions help control passage between adjacent endothelial cells, making them a major determinant of barrier selectivity. Changes in this junctional control can alter how readily substances move from blood toward tissue without requiring transport through the cells themselves. In neural tissue, this function contributes to the restrictive properties that protect the brain environment from many circulating substances.
Membrane transporters provide regulated pathways for particular substances to cross endothelial cells, whereas vesicular transport moves material through membrane-bound compartments within the cells. These mechanisms complement passage between cells and help shape the composition of exchange. Their activity is therefore relevant when determining whether a substance can reach neural tissue across specialized vascular endothelium.
Inflammatory signals can modify the endothelial barrier and change its selectivity. Such changes may increase or otherwise alter the movement of water, ions, solutes, or cells between blood and tissue. In neuroscience, examining this response helps connect vascular barrier behavior with neuroinflammation and with neurological conditions in which the protective properties of the blood-brain barrier may be disrupted.
A permeability assessment examines how selected water, ions, solutes, or cells move across an endothelial layer. Researchers can interpret the observed exchange in relation to transport through cells, passage between cells, vesicular transport, membrane transporters, junctional control, and inflammatory signaling. This approach provides information about barrier selectivity and whether endothelial behavior has changed under a studied condition.
Measurements can indicate how effectively specialized brain endothelium restricts circulating substances while allowing essential nutrients to reach neural tissue. Comparing movement across the endothelial layer helps identify changes in barrier selectivity and possible disruption. These outcomes are useful for studying neuroinflammation, cerebrovascular disease, and neurological disorders associated with altered vascular protection.
The blood-brain barrier limits entry of many circulating substances, creating an important constraint for delivering treatments to neural tissue. Permeability studies help characterize that constraint and identify how barrier behavior changes in disease-related settings. They also support research on neuroinflammation, cerebrovascular disease, barrier disruption, and approaches intended to understand or address restricted access to the brain.