In Vitro Model
In Vitro Model - Related Videos
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
08:03
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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Cited by 22 •
2017
Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.
08:41
Modeling Chemotherapy Resistant Leukemia In Vitro
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Cited by 15 •
2016
The current report summarizes a protocol that can be utilized to model the influence of the bone marrow microenvironment niche on leukemic cells with emphasis placed on enrichment of the most chemoresistant subpopulation.
10:13
Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
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Cited by 10 •
2015
We have developed an eye irritation test which utilizes a three dimensional reconstructed human cornea-like epithelial (RhCE) tissue model. The test is able to discriminate between ocular irritant and corrosive materials (GHS Categories 1 and 2 combined) and those that do not require labeling (GHS No Category).
10:29
Christopher Hughes: An in vitro model for the Study of Angiogenesis (Interview)
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Cited by 1 •
2007
Christopher C.W. Hughes describes the utility of his culture system for studying angiogenesis in vitro. He explains the importance of fibroblasts that secrete a critical, yet unidentified, soluble factor that allow endothelial cells to form vessels in culture that branch, form proper lumens, and undergo anastamosis.
FAQs
Controlled conditions let investigators change selected factors, such as genes, chemicals, or environmental conditions, while maintaining the neural system in defined media. This design makes it easier to connect a treatment or perturbation with changes in cellular signaling, electrical activity, survival, or cell interactions. The observations are also more reproducible because experiments occur in an accessible, standardized setting.
Each system preserves a different level or type of neural biology. Cultured neurons and glial cells support focused studies of individual cell behavior and interactions, whereas brain tissue slices retain features of tissue organization. Stem cell-derived neural systems provide another way to examine neural processes. Selecting among them depends on whether the study emphasizes cells, tissue context, development, or interactions.
Researchers can examine cellular signaling, electrical activity, survival, and interactions among neural cells or tissues. These measurements show how neural systems respond after controlled changes to genes, chemicals, or environmental conditions. Together, they can clarify how a perturbation affects function and viability, supporting studies of neuronal development, disease mechanisms, and responses to potential therapeutics.
A typical workflow maintains cultured neurons, glial cells, brain tissue slices, or stem cell-derived neural systems in defined media. Researchers then apply a controlled change involving genes, chemicals, or environmental conditions and examine the system afterward. They measure outcomes such as signaling, electrical activity, survival, or interactions, linking the experimental change to specific neural responses.
These systems are useful when researchers need to examine disease mechanisms under controlled conditions or evaluate how neural cells and tissues respond to potential therapeutics. Because investigators can introduce defined genetic, chemical, or environmental changes and measure specific outcomes, the models help connect experimental conditions with cellular or functional responses before broader research is pursued.
Stem cell-derived neural systems can extend neuroscience research toward personalized approaches by providing neural systems for controlled investigation, while cultured cells and tissue-based systems support studies of neural development and repair-related questions. Their accessible design also contributes to regenerative neuroscience and can reduce reliance on animal studies, while preserving measurements of signaling, activity, survival, and cellular interactions.