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Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

DOI:
10.3791/52979-v

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10:13 min

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August 23, 2015

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, , , ,
1MatTek Corporation, 2MatTek In Vitro Life Science Laboratories

Chapters

  • 00:05Title
  • 01:43Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Treatment Preparation (Day 0)
  • 03:08Pre-treatment and Test Article Exposure (Day 1)
  • 04:42Rinsing
  • 06:13MTT Viability Assay (Liquid Test Day 1; Solid Test Day 2)
  • 08:19Results: Representative EIT Results for 10 Test Articles
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed an eye irritation test which utilizes a three dimensional reconstructed human cornea-like epithelial (RhCE) tissue model. The test is able to discriminate between ocular irritant and corrosive materials (GHS Categories 1 and 2 combined) and those that do not require labeling (GHS No Category).

Tags

Keywords: Eye Irritation TestEITRhCEOcular ToxicityEU Cosmetics DirectiveEU REACHNon-animal Alternative MethodsLiquid ChemicalsSolid Test ArticlesMTT AssaySensitivitySpecificityAccuracyOECD Test GuidelinesTG 492

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