Neural Tube Formation
Neural Tube Formation - Related Videos
12:48
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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Cited by 26 •
2012
Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.
07:37
Neural Tube Closure in Mouse Whole Embryo Culture
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Cited by 22 •
2011
A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.
08:12
Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
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Cited by 345 •
2014
The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.
04:00
A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells
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Cited by 121 •
2011
A tube formation assay is used to evaluate vascular activity of tumor cells.
09:57
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
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Cited by 11 •
2015
Here we report dual labeling of neural crest cells and blood vessels using chickGFP neural tube intraspecies grafting combined with intra-vascular DiI injection. This experimental technique allows us to simultaneously visualize and study development of the NCC-derived (enteric) nervous system and the vascular system, during organogenesis.
FAQs
The notochord acts as an underlying signaling source that induces the overlying ectoderm to become a neural plate. This interaction establishes the initial tissue identity required for central nervous system development. Studying this induction step helps explain how early embryonic signals direct ectodermal cells toward brain and spinal cord precursors.
Neural Tube Formation depends on a sequence of coordinated shape changes rather than a single fusion event. The neural plate develops elevated edges, those edges bend toward one another, and they fuse to enclose the neural tube. This tissue remodeling provides a framework for examining how embryonic structure is converted into an organized nervous system precursor.
Neural crest cells detach from the forming neural tissue and migrate away, whereas the fused neural tube remains the precursor of the brain and spinal cord. Their separation and movement create distinct developmental outcomes from the same early remodeling process. This distinction is important when relating neurulation to both central and peripheral nervous system development.
Analysis of Neural Tube Formation reveals how coordinated embryonic tissue remodeling contributes to central nervous system patterning. Researchers can use this developmental context to connect early structural changes with the organization of future brain and spinal cord regions. The process therefore provides a foundation for investigating normal nervous system development as well as congenital abnormalities.
Errors in the coordinated events of neurulation can produce neural tube defects, including spina bifida and anencephaly. These conditions demonstrate that successful neural plate shaping, bending, and fusion are essential for normal nervous system development. Examining where development becomes disrupted helps link embryonic tissue behavior with congenital disease outcomes in neuroscience.
The process offers a developmental reference for understanding how nervous system precursors arise and become organized. Its study supports research into early brain development, congenital disease, and regenerative strategies by identifying the coordinated tissue changes that establish central nervous system precursors. These connections make neurulation relevant beyond embryology, particularly for interpreting developmental and repair-oriented neuroscience questions.