Name: recording a reproducible lab experiment
Uploaded: 2020-03-26T07:22:27.000Z
Duration: 3 min 6 s
Channel: JoVE

K12 Standards

Texas Essential Knowledge and Skills

High School

Specialised Topic in Science

112.51.c.1.

112.51.c.1.D.

Proper Lab Notebook Keeping

在实验室工作中，一个基本要求是使用实验记录本对实验进行准确且及时的记录。实验记录本在学术界和工业界都至关重要，因为它不仅能够提供所进行实验的详细记录，还可作为证明研究成果所有权和原创性的法律文件。
必须使用装订好的无碳复写实验记录本，以便将副本取下并提交给指导教师。记录时必须始终使用钢笔，不得使用铅笔。在开始实验之前，必须在实验记录本中完成实验前报告。
为此，您必须首先仔细阅读实验步骤，并明确实验目的。然后，写出理解该实验和方案所需的所有背景信息。
接下来，您必须撰写实验步骤，同时对复杂的步骤进行说明，并计算可能需要的数值。简单复制实验方案是不够的。必要时可绘制流程图以帮助您完成这一过程。
你还需要提前写出所有配平的化学反应方程式，确定理论产量，并明确所用各种材料的物理性质。可能最重要的一步是识别所有反应物、产物和溶剂的安全风险。这些危险性必须从每种物质的材料安全数据表（Material Safety Data Sheet，MSDS）中获取。
当您准备开始实验时，应首先记录下日期以及正在进行的实验名称。随后，如实记录实验过程中执行的每一步操作，并包含精确的测量数据，例如体积、温度和重量。实验记录本应始终放置在身边，并立即记录相关信息。切勿将笔记随意写在纸巾或零散纸张上。
如果出现错误，应使用单线划掉，并立即写上正确信息。务必始终记录所有相关观察结果，例如温度变化、颜色变化或pH值变化。必须包含所有观察结果和数据点，包括异常值和失败的实验。切勿删除任何页面或数据点以试图掩盖错误。
完成实验及所有记录工作后，划掉最后一页未使用的部分，并在所有页上签名并注明日期。在本实验中，你将通过进行苯甲酸甲酯的硝化反应来练习实验记录本的记录技能。实验过程中必须保持准确而完整的记录，以便在实验结束后能够正确分析实验结果。

规范的实验记录本维护

<h4>有机化学实验记录本的维护</h4>
记录是科学和有机化学实验室中的一项关键技能。如果没有适当的笔记、描述、数量或观察结果，实验室中进行的实验将无法被重复或再现。因此，保持一份准确且及时更新的实验记录本对于实现科学研究的可重复性至关重要。实验记录本在学术界和工业界都是一种强有力的工具。当某项发现被申请专利并实现商业化时，实验记录本尤为重要。在这种情况下，实验记录本是一份法律文件，能够证明某一想法、方法、物质或技术的所有权。
实验记录本中的关键信息包括所执行的步骤以及所有观察结果。实验目的必须表述清晰、书写明确，以便于理解。对于每个实验，实验记录本中需记录若干基本部分。实验报告由实验前准备、实验方案、观察结果与数据以及实验后总结组成。
建议使用装订成册且无散页的实验记录本。记录本的页面不应轻易被撕下，以免影响记录的完整性与可信度。对于需要评分的实验课程，应使用带有自复写功能纸张的记录本，以便您和指导教师各自保留一份实验记录本页面的副本。
所有记录必须用钢笔填写。任何可轻易擦除的书写工具均不可接受。所有记录必须是永久性的，以确保实验记录本中资料的有效性。所有笔记、观察结果、实验步骤的变更以及收集的数据都必须直接写入实验记录本中。切勿将笔记写在散页纸上，或在实验记录本中涂改记录。若记录本中的记录出现错误或需要修改，应使用单线整齐地划去错误内容，并在其旁边写上正确内容。涂改或撕毁页面会使记录无效，并违背准确记录的主要目的。当所有记录完成后，应将当页剩余空白区域用线划去。所有使用过的页面均须签名并注明日期。
<h4>实验前准备</h4>
实验前必须完成预实验报告。撰写预实验报告时，应仔细阅读实验步骤，查阅实验相关的背景知识，并使用描述性短语为实验命名。例如，“1,3-戊二醇的合成”比“实验1”更适合作为标题。报告中应注明实验操作的日期、你的姓名，以及必要时注明实验搭档的姓名。报告还必须包含实验目的，即列出实验的目标。接下来，写出实验中将发生的所有配平的化学反应方程式。以表格形式列出实验中使用或生成的化学品的任何相关性质，例如熔点、分子量和密度。在正式实验前完成所有必要的计算。
此外，需识别所有试剂的安全风险。这些信息包含在制造商提供的材料安全数据表（MSDS）中。计算产物的理论产量也十分重要，这有助于判断实验是否正常进行。最后，应在实验记录本中写出详细的分步操作步骤。所写内容应包含足够的描述性信息，以便他人能够重复该实验。因此，不应逐字照搬自其他来源的操作流程，而必须用自己的语言进行撰写。
<h4>实验室观察</h4>
本部分应包含实验过程中所有的观察记录和实验结果，因此必须在实验进行的同时实时填写。在此处记录所有相关观察结果，例如反应温度，以及实验过程中颜色或 pH 值的变化。反应物和产物的精确质量也需在此处记录。同时，务必记下实验过程中生成的任何计算机文件的名称。
<h4>实验后</h4>
实验后报告应呈现数据和最终分析结果。报告需包含您的假设或预期，并将其与实验结果进行比较。如果预期与观察结果存在差异，应对此进行讨论，并探讨可能导致差异的原因。还应讨论该技术的局限性，以及为获得更佳结果可进行的改进。最后，实验后报告应总结实验数据，给出明确的结论，并解释可能存在的误差。
<h4>参考文献</h4>
<ol>
	<li style="font-size:17px">Harris, D.C. (2015). 定量化学分析。纽约，纽约州：W.H. Freeman and Company。</li>
</ol>

有机化学实验记录本的维护  
记录是科学研究和有机化学实验中的一项关键技能。如果没有适当的笔记、描述、用量或……

Education

教育

JoVE Lab Manual

Chemistry

化学

正确保存实验室笔记本

recording a reproducible lab experiment

Accurate lab notebook records are essential in science and organic chemistry labs. Without clear notes, descriptions, quantities, and observations, an experiment cannot be repeated or checked. Good record keeping supports reproducibility, which means other people can follow the same work and get similar results.

A laboratory notebook is useful in school labs, research settings, and industry. It can also become a legal record when an idea, process, substance, or technique is patented and commercialized. For that reason, the notebook must clearly show what was done and what was observed during the experiment.

A strong lab notebook entry includes several parts. The full lab report usually contains a pre-lab, lab protocol, observations and results, and a post-lab. A bound notebook with no loose pages is preferred, and pages should not tear out easily. In graded lab classes, self-duplicating sheets are useful because both the student and the instructor can keep a copy.

All records should be written in pen. Erasable writing tools are not acceptable because the notes must stay permanent. Notes, observations, procedure changes, and data should go directly into the notebook. Do not use loose paper, and do not scribble out or remove records. If a mistake happens, cross it out neatly with one line and write the correction beside it. When the page is finished, cross out any blank space. Every used page should also be signed and dated.

The pre-lab is completed before the experiment begins. It starts with careful reading of the procedure and research on the experiment background. The title should be descriptive, such as a specific synthesis name instead of a label like Experiment 1. The pre-lab should also include the date, the student name, and the lab partner name if needed, along with the objective of the experiment.

The pre-lab also includes the balanced chemical reactions that will occur. Relevant properties of the chemicals, such as melting point, molecular weight, and density, are listed in a table. Any needed calculations should be done before the lab starts. Safety risks for each reagent must also be identified using the material safety data sheets, or MSDS, provided by the manufacturer. It is also important to calculate the theoretical yield of the product so you can judge whether the experiment is progressing as expected. Finally, the procedure should be written in the notebook in clear, step-by-step form and in your own words so someone else could repeat the work.

The lab observations section is filled out during the experiment. This is where you record temperature changes, color changes, pH changes, and other relevant observations as they happen. You also record the exact mass of reactants and products. If the experiment creates computer files, write down the file names as part of the record.

The post-lab report comes after the experiment and presents the data and final analysis. It compares the hypothesis or expectations with the actual results. If the results were different, the reasons should be discussed. The post-lab should also cover limits of the technique and possible improvements for better results. It ends with a clear summary of the data, a conclusion, and an explanation of any errors.

Recording a Reproducible Lab Experiment

lab techniques

Measuring with Accuracy in the Lab

Measuring with accuracy in the lab starts with careful records, the right tools, and correct reading of data. Scientists use lab notebooks to record how an experiment was done. These notes include the amounts of materials used and each step of the procedure. Without that record, an experiment cannot be repeated in the same way. Balances and glassware are used to measure solids and liquids for experiments. The precision of a measurement depends on the equipment used. Digital balances show the...

scientific measurement and lab skills

Graphs, Curve Fit, and Standard Deviation

Two-dimensional graphs help students compare an independent variable with a dependent variable in science data. In a chemistry example, temperature is the independent variable and volume is the dependent variable. After data are collected, the relationship between the two measurements must be quantified so it can be evaluated and compared with other relationships. A graph can also show when no relationship exists between the two variables. To make a two-dimensional graph, each data point needs...

stoichiometry, product yield, and limiting reactants

stoichiometry-product-yield-and-limiting-reactants

Balancing Equations and Reactant Limits

Chemical equations show how reactants change into products during a reaction. Stoichiometry is the study of the relative amounts of reactants and products. It is based on the Law of Conservation of Mass, which says matter is neither created nor destroyed. Because atoms are only rearranged, the number and identity of atoms must match on both sides of a reaction. That is why a chemical equation must be balanced. A balanced equation has the same number of atoms for each element in the reactants...

redox reactions

Oxidation States in Redox Reactions

Oxidation states help identify redox reactions. In a redox reaction, oxidation is the loss of one or more electrons, and reduction is the gain of one or more electrons. These reactions are often tracked by changes in oxidation state, also called oxidation number. Each atom in a molecule has an oxidation state. This number shows how oxidized an atom is compared with its free elemental form. It is written as the charge an atom would have if every bond were purely ionic. In that model, bonding...

ideal gas law

Gas Laws Behind the Ideal Gas Equation

The ideal gas equation brings together the main gas laws used in chemistry. Gases have molecules that are far apart, so their behavior is described with four measurable variables: pressure, volume, number of moles, and temperature. The ideal gas law links these variables with the gas constant, R, which equals 8.314 J·K−1 mol−1. Boyle’s law describes the link between pressure and volume. When temperature and the number of moles stay constant, pressure and volume change in opposite directions.

acid and base concentrations

Acids, Bases, and pH in Titration

Acids, bases, and pH explain how acidic or basic a solution is. An Arrhenius acid releases hydrogen ions when it dissolves in water, while an Arrhenius base releases hydroxide ions. These reactions also create conjugate partners: an acid forms a conjugate base after losing hydrogen, and a base forms a conjugate acid after losing hydroxyl. Every acid has a conjugate base, and every base has a conjugate acid. pH measures the amount of hydrogen ions in a solution. The pH scale runs from 0 to 14...

buffers

Buffer pH and the Common Ion Effect

Buffer solutions help keep pH steady when acids or bases are added. In water, an Arrhenius acid (HA) dissociates into hydrogen ions, H+, and a conjugate base, A-. More hydrogen ions make a solution more acidic and lower its pH. An Arrhenius base (BOH) dissociates into a conjugate acid, B+, and hydroxide ions, OH-. Strong acids and strong bases usually change pH a lot because they react completely in water and raise the amount of H+ or OH-. Buffers reduce those pH changes. A buffer usually...

enthalpy of reaction

Reaction Heat, Hess’s Law, and Calorimetry

Reaction heat is explained through enthalpy, Hess’s law, and calorimetry. These ideas show how heat moves during chemical reactions and how scientists measure it. Thermodynamics is the study of heat energy and other forms of energy, such as work. Its laws are used across science, from biology to physics. The first law says energy is conserved in an isolated system. Energy can change form, such as thermal energy becoming work, but it cannot be created or destroyed. The second law of...

solubility

Solubility in Water and Other Solvents

Solubility describes how much of a solute can dissolve in a specific solvent. It is usually reported as solute mass per solvent volume or as solute mass per solvent mass. For example, sodium chloride has a solubility of 36 g per 100 mL of water at room temperature. If solubility is given by solvent mass, that mass must be converted to volume for later calculations. Temperature changes solubility. Sodium carbonate dissolves at about 7 g per 100 mL of water at 0 °C, 22 g per 100 mL at room...

metal flame emission

Flame Test Metal Color Clues

Flame testing uses a metal sample and a flame to identify a substance by its color. Each element gives off a characteristic light emission in the flame, so the color can help distinguish one element from another. This same idea appears in fireworks, where different metal components create different colors. The color comes from energy absorbed by the metallic component. When a species absorbs energy, that energy can go into translation, vibration, rotation, or electron excitement. Electron...

balmer series

Hydrogen Emission Lines

Hydrogen emission lines show how electrons move between fixed energy levels in the Bohr model. Niels Bohr proposed this model for the hydrogen atom in 1913. In this model, an electron can only occupy certain orbits, and it cannot exist between them. These energy states are labeled with the quantum number n. The ground state is n = 1. Higher values, such as n = 2, 3, 4, and so on, are excited states. An electron changes state only by absorbing or emitting energy equal to the difference between...

beer's law

Absorbance Spectra and Beer-Lambert Analysis

Absorbance spectra and the Beer-Lambert Law show how light can be used to study molecules in solution. When light hits a substance, it may be absorbed, transmitted, or reflected. A substance often interacts with a range of wavelengths, and each wavelength can affect the molecules or atoms in a different way. When a molecule absorbs light, that energy can be used for translation, vibration, rotation, or electron excitement. Electron excitement is especially important in spectroscopy because it...

concentration dependence

Reaction Order and Rate Constants

Chemical kinetics describes the speed of a chemical reaction. The rate depends on the reaction mechanism, the complexity of the reaction, and the number of reactants involved. Reactant concentration also affects how fast a reaction happens. The rate law is the experimental relationship that connects these factors to reaction rate. In the rate law, each reactant contributes through a reaction order. The general form is r = k[A]^m[B]^n for aA + bB + ... → cC. Here, r is the reaction rate, k is...

temperature dependence

Reaction Rate, Arrhenius, and Catalysts

Chemical kinetics explains how fast a reaction happens and why that speed changes. The reaction rate is the speed of a chemical reaction. It is defined as the change in concentration of a reactant or product over time. Reaction speed depends on several factors, including the concentration of the reactants and the temperature of the reaction. Each reactant affects the rate in a specific way. That relationship is described by the rate law. The rate law is an equation that connects the...

galvanic cells

Battery Power from Redox Reactions

Electrochemistry studies how chemical change and electrical energy are linked. In this branch of chemistry, electrons move from one species to another. That movement can either produce current or be driven by an applied current. The main reaction in electrochemistry is the oxidation-reduction, or redox, reaction. A redox reaction has two half-reactions. Oxidation is the loss of electrons, and reduction is the gain of electrons. These changes also shift the oxidation state of each substance. The...

electrolytic cells

Electroplating with Electrolytic Cells

Electrochemistry links electrical energy with chemical change, and electrolytic cells show how electricity can drive a nonspontaneous reaction. In these reactions, electrons move from one species to another. The key chemistry is a redox reaction, or oxidation-reduction reaction, which happens in two half-reactions. Oxidation is the loss of electrons, and reduction is the gain of electrons. The substance that loses electrons is the reducing agent, and the substance that gains electrons is the...

proper lab notebook keeping

Accurate lab notebook records are essential in science and organic chemistry labs. Without clear notes, descriptions, quantities, and observations, an experiment cannot be repeated or checked. Good record keeping supports reproducibility, which means other people can follow the same work and get similar results. A laboratory notebook is useful in school labs, research settings, and industry. It can also become a legal record when an idea, process, substance, or technique is patented and...

basic organic chemistry techniques

Organic Lab Glassware and Safe Handling

Organic chemistry labs use specific glassware, careful weighing steps, and controlled heating to keep reactions safe and accurate. Beakers and Erlenmeyer flasks are often used for mixing or holding solvents, but they are not meant for measuring volume unless only an approximate amount is needed. Volumetric glassware is used when a precise volume matters. This group includes graduated cylinders, volumetric pipettes, and volumetric flasks. A graduated cylinder is commonly used in the organic lab...

melting points

How Impurities Change Melting Points

Melting point helps identify an organic compound by showing the temperature where a solid turns into a liquid at 1 atmosphere of pressure. It is a physical property, like solubility, density, color, and electronegativity. Because the exact start of melting is hard to measure, the result is reported as a range. The lower end is when the first drops of liquid appear. The upper end is when the last solid has melted. Scientists compare this range with literature values to help identify a compound. <br...

boiling points

Boiling Point and Vapor Pressure

Boiling point and vapor pressure are key physical properties of a liquid. For a pure compound, the boiling point can help identify the substance. In experiments, the boiling point is usually recorded as a range because exact values are hard to measure and may differ by a few degrees from the literature value. Vapor pressure explains why a liquid boils. In a sealed container, some molecules at the liquid surface gain enough energy to escape into the gas phase. At the same time, some gas...

recrystallization

Purifying Crystals by Solubility

Purifying crystals by solubility is a key organic chemistry technique. Recrystallization uses differences in solubility to separate a desired solid product from starting materials, solvent, and impurities. It is one of the three standard purification methods, along with distillation and extraction. Solubility is the maximum amount of a substance that can dissolve in a fixed volume of solvent at a given temperature. Different solutes dissolve in different solvents, and their behavior can vary...

extraction

Separating Mixtures with Solvent Extraction

Solvent extraction is a common way to separate and isolate a target compound in organic chemistry. In this process, a solute moves from one phase to another so it can be separated from unreacted starting materials or impurities. Extraction is also useful when a product is dissolved in a reaction solvent that is hard to remove by evaporation, such as a solvent with a high boiling point. There are three main types of extraction. In solid-liquid extraction, the solute moves from a solid phase...

simple distillation

Separating Liquids by Boiling Point

Separating liquids by boiling point is the goal of distillation. A miscible mixture contains liquids that mix evenly, and it can be heated so that the more volatile liquid evaporates first. Volatility is a liquid’s tendency to vaporize, and boiling begins when bubbles form in the liquid phase. In a closed container, liquid and vapor can reach equilibrium, which means the rate of vaporization equals the rate of condensation. The pressure from the vapor above the liquid is called vapor pressure.

steam distillation

Steam Distillation and Low-Boiling Separation

Steam distillation is a separation method for temperature-sensitive organic compounds. It works best when the organic compound is immiscible in water, meaning the two liquids do not mix. The method helps remove the organic molecule from a non-volatile contaminant without using the higher temperatures needed for many other separations. In steam distillation, the immiscible mixture is heated until both water and the volatile organic compound distill together. The vapor rises to a condenser,...

thin-layer chromatography

TLC Separation by Polarity

Thin-layer chromatography (TLC) separates compounds in a mixture by polarity. It uses the same basic parts as other chromatography methods: a stationary phase, a mobile phase, and the solute. In TLC, the stationary phase is a thin coating on a plate instead of material packed into a column. The stationary phase in most TLC systems is polar silica gel, a form of silicon dioxide. Its surface has OH groups that can form hydrogen bonds. The sample is first spotted on a pencil start line with a...

column chromatography

Separating Mixtures by Polarity

Column chromatography separates compounds in an organic chemistry lab by using differences in physical properties. It is especially useful for microscale separations when less than 10 grams of material is available for analysis. The method can separate a mixture based on solubility, polarity, hydrophobicity, size, and charge. Any chromatography system has three main parts: the stationary phase, the mobile phase, and the sample mixture. In column chromatography, the stationary phase is usually...

hydrolysis of an ester

Soap Formation from Triglycerides

Triglycerides are lipids made of a glycerol backbone linked to three fatty acids. They are common in vegetable oils and animal fats. Plant lipids are the basis of vegetable oils, while animal lipids are used as lard or general sources of fats. A fatty acid has a long carbon chain, usually between 12 and 20 carbons, with a weak organic acid at one end. Some fatty acids contain double bonds. These are unsaturated fats. The double bond usually gives the chain a bent, cis shape. As a result,...

synthesis of luminol

Luminol Chemiluminescence in Forensics

Luminol chemiluminescence shows how a chemical reaction can produce light and help with blood detection. Chemical reactions may be endothermic or exothermic, depending on whether they absorb or release heat as reactants become products. The enthalpy of a reaction is the energy difference between reactants and products, or ΔH. A positive ΔH means the reaction is endothermic. A negative ΔH means it is exothermic. Many reactions do not happen in a single step. They can pass through intermediates...

esterification

Ester Formation and Everyday Fragrances

Esters are organic compounds with a carbonyl group attached to an oxygen that connects to another alkyl or aryl group. Their general formula is RCOOR'. They are often made by reacting a carboxylic acid with an alcohol in an esterification reaction. In this process, the hydroxyl group of the acid is replaced by an alkyl or aryl group. Many simple esters have familiar fruit and flower smells because their structures are small and less complex. Formic acid and methanol can combine to form methyl...

identification of unknown aldehydes and ketones

identification-of-unknown-aldehydes-and-ketones

Carbonyl Tests for Aldehydes and Ketones

Aldehydes and ketones are carbonyl compounds, which means they contain a C=O functional group. A ketone has two alkyl or aryl groups attached to the carbonyl carbon. Acetone is the simplest ketone, with two methyl groups. An aldehyde is similar, but it has at least one hydrogen attached to the carbonyl carbon. Formaldehyde is the simplest aldehyde, and acetaldehyde has one hydrogen and one methyl group. The carbonyl carbon in both aldehydes and ketones is electrophilic because the oxygen atom...

uv-vis spectroscopy of dyes

How Dyes Absorb Visible Light

Dyes absorb visible light because their molecular structure lets them interact with specific wavelengths. When light hits a substance, some light is absorbed and the rest is reflected or transmitted. The color we see depends on the wavelength that is reflected. A blue substance reflects blue light, around 430 to 480 nm, and absorbs the complementary orange region, around 590 to 630 nm. Compounds that do not absorb in the visible range appear colorless to the human eye. Light also has energy and...

identifying alcohols

Alcohol Tests for Primary, Secondary, and Tertiary

Alcohol tests can help sort alcohols by structure and reactivity. Alcohols are organic molecules with a hydroxyl group attached to an alkyl or aryl group, written as ROH. If the carbon with the hydroxyl group has one R group, the alcohol is primary. If it has two R groups, it is secondary. If it has three R groups, it is tertiary. Alcohols can also be aromatic when they contain a benzene ring. Phenol is the simplest aromatic alcohol. To identify an alcohol, chemists can use its chemical...

chemistry

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The Scientific Method

The scientific method is a detailed, empirical problem-solving process used by biologists and other scientists. This iterative approach involves ...

Inductive Reasoning

Inductive reasoning is a form of logical thinking that uses related observations to arrive at a general conclusion. It is uncertain and operates in ...

Deductive Reasoning

Deductive reasoning, or deduction, is the type of logic used in hypothesis-based science. In deductive reasoning, the pattern of thinking moves in the ...

Correlation and Causation

Correlation and CausationStatistical tests can calculate whether there is a relationship, or correlation, between independent and dependent variables. A ...

Ask questions and define problems related to specialized topics of study based on observations or information from text, phenomena, models, or investigations;

112.51.c.1.A.

Scientific Method

The scientific method is used to solve problems and explain phenomena. The development of the scientific method coincided with changes in philosophy ...

Demonstrating the Scientific Method with Tree Branches
ExpandObtain branches containing a few dozen typical leaves from five different local tree species. ...

Observation and Background Research
ExpandNOTE: The first two steps in the scientific process involve studying and observing natural phenomena of ...

Problem-Solving

A problem-solving strategy is a plan of action used to find a solution. It involves trial and error, algorithms, heuristics, and insights. Effective ...

Problem Definition

Problem definition is the initial and the most essential step in research.
The researcher identifies and explains the specific issue or gap to be ...

Apply science practices related to specialized topics of study to plan and conduct investigations or use engineering practices to design solutions to problems;

112.51.c.1.B.

Proper Personal Protective Equipment

Robert M. Rioux &#38; William A Elliott, Pennsylvania State University, University Park, PA
Hazards are many and varied in the laboratory, but the right ...

Emergency Eyewash and Shower Stations

Robert M. Rioux, Pennsylvania State University, University Park, PA
The Occupational Safety and Health Administration (OSHA) mandates that an emergency ...

Electrical Safety

Robert M. Rioux &amp; Suprita Jharimune, Pennsylvania State University, University Park, PA
Among the many hazards present in the laboratory, electrical ...

Working with Centrifuges

Source: Robert M. Rioux &amp; Suprita Jharimune, Pennsylvania State University, University Park, PA
Earth's gravitational force is capable of separating ...

Working with Hot and Cold Sources

Source: Robert M. Rioux &amp; Suprita Jharimune, Pennsylvania State University, University Park, PA
Working with extreme temperatures, both high and low, ...

guidelines-in-case-of-a-laboratory-emergency

Guidelines in Case of a Laboratory Emergency

Robert M. Rioux &amp; Zhifeng Chen, Pennsylvania State University, University Park, PA
The most common laboratory emergencies include chemical spills, ...

chemical-storage-categories-hazards-and-compatibilities

Chemical Storage: Categories, Hazards And Compatibilities

Source: Robert M. Rioux &amp; Taslima A. Zaman, Pennsylvania State University, University Park, PA
While the use of various chemicals in experimental ...

Safe Handling of Mineral Acids

Source: Robert M. Rioux &#38; Taslima A. Zaman, Pennsylvania State University, University Park, PA
A mineral acid (or inorganic acid) is defined as a ...

Handling Chemical Spills

Source: Robert M. Rioux &#38; Taslima Zaman, Pennsylvania State University, University Park, PA
Since chemicals are commonly used for laboratory research ...

Proper Use of Autoclaves

Robert M. Rioux &amp; Zhifeng Chen, Pennsylvania State University, University Park, PA
Autoclaving is one of the most commonly used methods in the ...

Fume Hoods and Laminar Flow Cabinets

Robert M. Rioux &amp; William A. Elliott, Pennsylvania State University, University Park, PA
Fume hoods and laminar flow cabinets are engineering controls ...

Decontamination for Laboratory Biosafety

Robert M. Rioux and Zhifeng Chen, Pennsylvania State University, University Park, PA
Decontamination is essential for laboratory biosafety, as the ...

Proper Waste Disposal

Robert M. Rioux and Taslima A. Zaman, Pennsylvania State University, Pennsylvania, PA

Users are responsible for the proper disposal of the waste ...

Use appropriate safety equipment and practices during laboratory, classroom, and field investigations as outlined in Texas Education Agency-approved safety standards;

112.51.c.1.C.

Common Lab Glassware and Uses

Source: Laboratory of Dr. Neal Abrams &#8212; SUNY College of Environmental Science and Forestry
Glassware is a regular appearance in the professional ...

Lab Techniques

Measurement Accuracy
The ability to repeat an experiment and get the same results, or reproducibility, is essential in scientific research. However, ...

Source: Smaa Koraym at Johns Hopkins University, MD, USA

	Preparation of LaboratoryExpand
	Here we show the laboratory preparation for 10 students ...

Source: Smaa Koraym at Johns Hopkins University, MD, USA

	Determining the Density of an EggExpand
	Density is a measure of how compact a substance or ...

Organic Lab Notebook Keeping
Record keeping is a vital skill in science and organic chemistry labs. Without proper notes, descriptions, quantities, or ...

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA

	Preparation of the LaboratoryExpand
	Here, we show the laboratory preparation ...

Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA

	Nitration of Methyl BenzoateExpand
	From this lab onward, ...

Use tools appropriate to the specialized topic of study;

SI Units: 2019 Redefinition

Measurement is integral to analytical chemistry. Each record comprises a number &#8211; denoting the magnitude&#160; &#8211; and the unit &#8211; a ...

International System of Units

The International System of Units, known as the SI system, is a universally accepted measurement system recognized and used worldwide. The SI system is ...

Measurement: Standard Units

Every measurement provides three kinds of information: the size or magnitude of the measurement (a number), a standard of comparison for the measurement ...

Measurement: Derived Units

The International System of Units or SI system, by international agreement, has fixed measurement units for seven fundamental properties: length, mass, ...

Collect quantitative data using the International System of Units (SI) or qualitative data as evidence as appropriate to the specialized topic of study;

112.51.c.1.E.

Review and Preview

Data are individual items of information obtained from a population or sample. Data may be classified as qualitative (categorical), quantitative ...

What is a Frequency Distribution

A frequency is the number of times a value of the data occurs. The sum of all the frequency values represents the total number of students included in the ...

Organize quantitative or qualitative data using representations appropriate to the specialized topic of study such as scatter plots, line graphs, bar graphs, charts, data tables, diagrams, scientific drawings, and student-prepared models;

112.51.c.1.F.

Scientific and engineering practices. The student, for at least 40% of instructional time, asks questions, identifies problems, and plans and safely conducts classroom, laboratory, and field investigations to explain phenomena or design solutions using appropriate tools and models. The student is expected to:

Recording a Reproducible Lab Experiment

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