Name: luminol chemiluminescence in forensics
Uploaded: 2020-03-26T07:22:29.000Z
Duration: 3 min 2 s
Channel: JoVE

Ordinarily, when reactants change to products, the difference in their energy is known as the enthalpy of the reaction, which can be either exothermic, where heat is released, or endothermic, where heat is absorbed.
In a chemiluminescent reaction, the reactants form an intermediate, which is in an electronically excited state. This intermediate is unstable. As it relaxes down to the ground state, the electrons in the intermediate releases energy in the emission of visible light rather than in the form of heat.
This phenomenon is similar to fluorescence. Recall that the electrons in a molecule occupy the ground state. When they absorb energy, such as when exposed to a certain wavelength of light, they become excited and transition to a higher, excited energy level. As the excited state relaxes back down to the ground state, the excess energy is emitted in the form of visible light.
However, it is important to distinguish between the two. The difference is how the electrons of the molecules are excited. In chemiluminescence, energy generated from a chemical reaction excites the electrons&#8230;whereas in fluorescence, the electrons are excited by direct absorption of light energy or other electromagnetic radiation. As the electrons relax down to ground state, both types of luminescence release the energy as emitted light.
Let&#8217;s take a look at an example of each. When you shine UV light on a bottle of tonic water, the electrons in quinine absorb the energy and are excited to a higher energy level. When the electrons relax, they release energy in the form of blue light.
Now, consider a glow stick, which has an outer tube containing diphenyl oxalate and a dye, and an inner tube containing hydrogen peroxide. When you bend the glow stick, the inner tube breaks, allowing the reactants to mix. The hydrogen peroxide oxidizes the diphenyl oxalate to form phenol and cyclic peroxide. The cyclic peroxide decomposes to carbon dioxide, releasing energy that excites the dye to a higher energy level. As the dye relaxes to the ground state, a photon of light is released. Here, the process is chemiluminescence because the energy provided to excite the dye comes from a chemical reaction.
In this lab, you will synthesize 3-aminophthalhydrazide, also known as luminol, by performing a series of reactions. You will then react the luminol with dimethyl sulfoxide over solid potassium hydroxide to observe its chemiluminescence.

synthesis of luminol

Chemical reactions can be classified as endothermic or exothermic depending on whether the reactions absorb or release heat when they change to products. The difference in energy between reactants and products is known as the enthalpy of the reaction. This is found by calculating the difference in energies between the products and the reactants, or &#916;H. If &#916;H is positive, the reaction is endothermic; if it is negative, the reaction is exothermic. While chemical reactions are traditionally thought of as a conversion of reactants into products, many reactions take place in multiple steps, forming intermediates. In chemiluminescent reactions, these intermediates transition from a high energy state to the ground state, releasing a photon of light that may be visible as the reaction progresses.
<h4>Atomic Energy Levels</h4>
Niels Bohr, a Danish physicist, proposed the theory that an electron orbiting the nucleus of the atom can occupy only certain orbits or energy levels. An atom with all of its electrons in the lowest possible energy level is said to be at its ground state. When an electron occupies an energy level higher than the ground state, the atom is in its excited state.
A key assumption in Bohr&#8217;s theory is that an electron remains in the ground state until it is disturbed. Thus, an electron is only elevated to an excited state by absorbing energy. When the electron relaxes back down to the ground state, it releases that energy. Often, this is in the form of a photon of light, whose wavelength is directly related to the energy difference between the excited and ground states.
When an electron is excited to a higher energy level by absorbing light of a certain energy or wavelength, the phenomenon is called fluorescence. This effect can be seen if you expose a laundered white T-shirt to a black light or UV-light. The characteristic glow is caused by the fluorescence of the detergent and bleaching compounds in the shirt, which make it look white under regular light.
<h4>Chemiluminescence</h4>
In a chemiluminescent reaction, an electron is excited by absorbing heat that is released during a reaction. Then, light is released when the electron relaxes back down to the ground state. The major difference between chemiluminescence and fluorescence is that the energy that excites the electrons in chemiluminescence comes directly from the reaction.
One practical application of chemiluminescence is in commercial glow sticks. A glow stick contains two separate solutions: one containing peroxide and the other containing diphenyl oxalate and a color dye. When the two solutions are mixed, which occurs upon activating the glow stick, the resulting reaction between the peroxide and diphenyl oxalate produces energy that excites the dye to a higher energy state. As the reactants are exhausted, the dye returns to the ground state and releases light. This is what gives glow sticks their characteristic color.
<h4>Luminol</h4>
Luminol is a chemical that exhibits chemiluminescent properties and is utilized in a wide range of applications, most notably in forensics. Luminol (C8H7N3O2) emits blue when it is mixed with an oxidizing agent. In the case of forensics, luminol reacts with the iron in hemoglobin, enabling forensic scientists to identify very small traces of blood.
Luminol is synthesized by the dehydration reaction of 3-nitrophthalic acid with hydrazine. The reaction is heated to remove water, and triethylene glycol is added to further increase the temperature. The nitro group of the 3-nitrophthalhydrazide is then reduced using sodium dithionite to form an amino group at high pH. In basic conditions, the 3-nitrophthalhydrazide is soluble. The addition of glacial acetic acid precipitates the luminol.
With potassium hydroxide, luminol forms a dianion. The hydroxide anions deprotonate the two hydrogens that are attached to the nitrogens in the luminol. Oxygen gas oxidizes luminol to its excited state. As it relaxes back to the ground state, it releases a blue-white light.
<h4>References</h4>
<ol>
	<li style="font-size:17px">Kotz, J.C., Treichel Jr, P.M., Townsend, J.R. (2015). Chemistry and Chemical Reactivity. Belmont, CA: Brooks/Cole, Cengage Learning.</li>
</ol>

Synthesis of Luminol

Chemical reactions can be classified as endothermic or exothermic depending on whether the reactions absorb or release heat when they change to products. ...

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luminol chemiluminescence in forensics

Luminol chemiluminescence shows how a chemical reaction can produce light and help with blood detection. Chemical reactions may be endothermic or exothermic, depending on whether they absorb or release heat as reactants become products. The enthalpy of a reaction is the energy difference between reactants and products, or ΔH. A positive ΔH means the reaction is endothermic. A negative ΔH means it is exothermic.

Many reactions do not happen in a single step. They can pass through intermediates along the way. In chemiluminescent reactions, these intermediates move from a high-energy state to the ground state and release a photon of light. That light can be seen as the reaction continues.

Atomic energy levels help explain why this glow happens. Niels Bohr proposed that electrons can occupy only certain orbits, or energy levels, around the nucleus. An atom is in the ground state when its electrons are in the lowest possible energy level. When an electron moves to a higher level, the atom is in an excited state. The electron must absorb energy to move up, and it releases that energy when it falls back down. This release is often a photon of light, and the light wavelength depends on the energy gap. When light causes this kind of excitation, the process is called fluorescence, such as the glow seen under a black light on a white T-shirt washed with detergent and bleach compounds.

Chemiluminescence is related to fluorescence, but the energy comes from the chemical reaction itself. In glow sticks, one solution contains peroxide and the other contains diphenyl oxalate and a color dye. When the solutions mix, the reaction produces energy that excites the dye. As the reactants are used up, the dye returns to the ground state and gives off light. This is why glow sticks have their characteristic color.

Luminol is a chemiluminescent chemical with an important forensic use. It emits blue light when mixed with an oxidizing agent. In forensic science, luminol reacts with the iron in hemoglobin, which helps detect very small traces of blood. Luminol is made by dehydrating 3-nitrophthalic acid with hydrazine. The reaction is heated to remove water, and triethylene glycol is added to raise the temperature further. Next, the nitro group in 3-nitrophthalhydrazide is reduced with sodium dithionite to form an amino group at high pH. The compound is soluble in basic conditions, and glacial acetic acid is added to precipitate the luminol. With potassium hydroxide, luminol forms a dianion because hydroxide ions remove the two hydrogens attached to the nitrogens. Oxygen gas then oxidizes luminol to its excited state. When it relaxes back to the ground state, it gives off a blue-white light.

Luminol Chemiluminescence in Forensics

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Luminol Chemiluminescence in Forensics

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