Barrier Function Assessment
Barrier Function Assessment - Related Videos
07:18
Oral Gavage in Neonatal Mouse Pups and Functional Assessment of Gut Barrier Integrity Using Ussing Chambers
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2026
This article describes: (1) oral gavage delivery of test molecules to neonatal mice as early as DOL 6; and (2) ex vivo evaluation of colonic epithelial barrier function in biopsies from DOL 10 to weaning (DOL 20) pups by assessing paracellular and transcellular permeability with Ussing chambers.
12:30
Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
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Cited by 213 •
2014
This protocol reviews Electric Cell-substrate Impedance Sensing, a method to record and analyze the impedance spectrum of adherent cells for the quantification of cell attachment, proliferation, motility, and cellular responses to pharmacological and toxic stimuli. Detection of endothelial permeability and assessment of cell-cell and cell-substrate contacts are emphasized.
11:41
Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
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2015
Measuring the barrier to the interspecies transmission of prion diseases is challenging and typically involves animal challenges or biochemical assays. Here, we present an in vitro prion protein conversion assay with the ability to predict species barriers.
03:01
Assessing Blood-Brain Barrier Integrity in a Laser-Induced Brain Injury Rat Model
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2025
Source: Kuts, R. et al. Laser-Induced Brain Injury in the Motor Cortex of Rats. J. Vis. Exp. (2020)This video demonstrates assessing blood-brain barrier (BBB) integrity following laser-induced brain injury in rats. A tracking dye is injected into the tail vein, allowing it to circulate to the brain and leak into the tissue through the damaged BBB. The brain is then extracted and processed to release the dye. The fluorescence intensity of the dye is measured to quantify the extent of...
10:36
In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption
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Cited by 6 •
2017
Maintaining blood-brain barrier coverage is key for the homeostasis of the central nervous system. This protocol describes in vitro techniques to delineate the fundamental and pathological processes that modulate blood-brain barrier coverage.
FAQs
Researchers often pair permeability measurements with electrical and ionic readouts because each probes a different aspect of barrier behavior. Molecular tracers indicate whether substances cross the cell layer, whereas transepithelial electrical resistance reports electrical properties associated with ion movement. Using these measures together can help connect transport changes to altered barrier function rather than relying on a single signal.
Barrier behavior may shift with cell differentiation, inflammation, mechanical stress, drug exposure, or the biomaterial used to construct the model. These factors can alter how epithelial or endothelial layers control tracer, ion, and solute movement. Comparing measurements across such conditions helps researchers evaluate whether an engineered interface responds appropriately to a biological challenge or design change.
Transport data alone show how substances move across a model, but they do not fully explain the state of the engineered tissue. Linking permeability or electrical findings with structural and functional properties provides a broader interpretation of barrier performance. This combined view helps distinguish meaningful changes in the biological interface from an isolated shift in one measurement.
A typical workflow begins with an engineered epithelial or endothelial model, followed by selection of a suitable readout such as molecular tracer permeability, transepithelial electrical resistance, or ion and solute passage. Researchers then monitor transport behavior under the condition of interest, such as drug exposure or mechanical stress, and compare the resulting measurements with the model's baseline or control condition.
The assessment is useful when researchers need to determine whether an engineered biological interface reproduces relevant transport behavior. In bioengineering, it supports evaluation of organ-on-chip systems, disease models, delivery platforms, and engineered tissues. Measurements can reveal how closely these systems model a physiological interface and how their barrier properties respond to drugs, inflammation, or material-related changes.
It can show whether a constructed epithelial or endothelial interface restricts or permits movement in a manner consistent with the intended model. Changes in tracer permeability, electrical resistance, or ion and solute passage provide functional evidence for evaluating the system. Researchers can use those outcomes to refine disease models, delivery platforms, organ-on-chip designs, and engineered tissues.