Tumor Perfusion
Tumor Perfusion - Related Videos
09:00
Spatial Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors
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Cited by 6 •
2016
The heterogeneous intra-tumoral accumulation of liposomes has been linked to an abnormal tumor microenvironment. Herein methods are presented to measure tumor microcirculation by perfusion imaging and elevated interstitial fluid pressure (IFP) using an image-guided robotic system. Measurements are compared to the intra-tumoral accumulation of liposomes, determined using volumetric micro-CT imaging.
04:24
Perfusion and Inflation of the Mouse Lung for Tumor Histology
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Cited by 34 •
2020
The purpose of this method is to present a simple and efficient method for the perfusion, inflation, and fixation of mouse lungs for the examination of lung tumor pathology and evaluation of metastases to the lung.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
08:47
Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
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Cited by 12 •
2012
The novel protocol reported in the present study allows selective detection of lung metastases at single cell resolution in mice by combined in-situ lung perfusion and fixation and X-Gal staining of lacZ-tagged tumor cells.
11:23
An Open-Source Normothermic Perfusion System Designed for Research Scientists
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Cited by 2 •
2025
Here, we present a protocol for an open-source, modifiable normothermic ex vivo perfusion system designed to overcome the high cost and limited flexibility of existing platforms, enabling more accessible, cost-efficient, and iterative use in pre-clinical biomedical research.
FAQs
Tumor-driven angiogenesis creates vessels that are irregular and leaky, rather than providing evenly distributed flow. This architecture produces uneven blood movement, so some regions receive less oxygen and nutrients while waste removal and therapeutic transport become inconsistent. In turn, this vascular disorder helps explain why treatment response can vary across different parts of the same tumor.
When blood flow is uneven, poorly supplied regions can become hypoxic, meaning oxygen availability is low. At the same time, elevated interstitial pressure makes it harder for therapeutic agents to move through tumor tissue. Considering both conditions helps bioengineers interpret limited penetration as a microenvironmental transport problem, not simply as a failure of the treatment itself.
Blood circulation through a tumor can vary because angiogenic vessels produce uneven flow across tissue. Consequently, oxygenation, nutrient supply, waste removal, and access for therapeutic agents may differ between regions or change as the tumor microenvironment changes. Treating perfusion as dynamic encourages measurements and models that capture spatial variation and treatment response instead of relying on a single uniform value.
Imaging provides a way to assess perfusion in tumor tissue by examining blood delivery and circulation. These measurements can help evaluate oxygenation-related conditions, identify unevenly supplied regions, and relate vascular behavior to treatment response. In bioengineering, imaging also complements engineered models, allowing researchers to compare observed vascular function with behavior reproduced in controlled experimental systems.
These models let researchers study vascular function under engineered conditions while examining how the tumor microenvironment shapes transport. Microfluidic and three-dimensional platforms can investigate drug penetration, reproduce relevant perfusion behavior, and evaluate treatment response in a controlled setting. Such systems support bioengineering strategies aimed at improving therapeutic delivery without depending only on measurements from tumors.
Perfusion measurements can connect vascular behavior with three practical outcomes: how far therapeutic agents penetrate, how a tumor responds to treatment, and whether delivery strategies improve access to poorly supplied tissue. They also reveal how the tumor microenvironment influences disease progression. This makes perfusion relevant to evaluating therapies and designing engineered approaches that address delivery limitations.