Adipogenic Progenitors
Adipogenic Progenitors - Related Videos
12:55
Purification of Progenitors from Skeletal Muscle
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Cited by 31 •
2011
Method for the enzymatic dissociation, surface labeling and purification by flow cytometry of fibro/adipogenic and myogenic progenitors from murine skeletal muscle.
09:49
Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
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Cited by 7 •
2021
This protocol outlines a method to isolate Fibro-adipogenic progenitors (FAPs) and myogenic progenitors (MPs) from rat skeletal muscle. Utilization of the rat in muscle injury models provides increased tissue availability from atrophic muscle for the analysis and a larger repertoire of validated methods to assess muscle strength and gait in free-moving animals.
07:00
Fibro-Adipogenic Progenitor Isolation, Expansion, and Differentiation from the Spiny Mouse Model
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2024
This protocol outlines the isolation of skeletal and cardiac muscle fibro-adipogenic progenitors (FAPs) from spiny mouse (Acomys) via enzymatic dissociation and fluorescence-activated cell sorting. The FAPs obtained from this protocol can be effectively expanded and differentiated to myofibroblasts and adipocytes.
11:25
FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
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Cited by 7 •
2022
The precise identification of fibro-adipogenic progenitor cells (FAPs) and muscle stem cells (MuSCs) is critical to studying their biological function in physiological and pathological conditions. This protocol provides guidelines for the isolation, purification, and culture of FAPs and MuSCs from adult mouse muscles.
10:16
Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
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Cited by 5 •
2017
Marrow stromal cells (MSCs) with neural potential exist within the bone marrow. Our protocol enriches this population of cells via hypoxic preconditioning and thereafter directs them to become mature Schwann cells.
FAQs
Their progression follows a coordinated sequence rather than a single event. Adipogenic progenitors first proliferate, then commit to the adipocyte lineage, and subsequently accumulate lipid as differentiation proceeds. Local signals and transcriptional programs help regulate these transitions, allowing researchers to distinguish changes in cell number, lineage commitment, and maturation during adipose tissue development.
Local signals and the surrounding tissue environment influence whether adipogenic progenitors continue proliferating, commit to the adipocyte lineage, or proceed through differentiation. This context helps explain why adipose tissue can respond differently under distinct biological conditions. Examining environmental effects therefore connects cell-level behavior with tissue development, expansion, and remodeling.
Metabolic state is one factor that can influence adipogenic progenitor behavior and the resulting formation of adipocytes. Changes associated with health or obesity may affect how adipose tissue develops, expands, or remodels, although the specific response depends on local conditions and regulatory programs. Studying these relationships helps connect progenitor biology with metabolic disease research.
These stages represent different biological outcomes and should not be treated as interchangeable. Proliferation changes the number of progenitor cells, commitment establishes adipocyte-lineage identity, and lipid accumulation marks progression through differentiation. Separating them helps researchers determine which part of adipose tissue development or remodeling is affected by local signals, transcriptional regulation, or metabolic state.
Researchers can follow how these cells proliferate, commit to the adipocyte lineage, and accumulate lipid during differentiation. They can also consider how local signals, tissue environment, and metabolic state influence those outcomes. Together, these observations provide a framework for studying adipose tissue development, expansion, and remodeling without reducing the process to lipid storage alone.
Adipogenic progenitors provide a way to investigate how adipose tissue changes during health and obesity. Their behavior can be examined in relation to tissue development, expansion, and remodeling, helping clarify cellular processes associated with metabolic disease. This research connects progenitor regulation with broader questions about how adipose tissue responds to altered metabolic conditions.
These cells are relevant because their differentiation can support controlled formation of functional fat cells. In adipose tissue engineering, researchers can use their staged progression as a framework for studying tissue formation. Regenerative strategies likewise benefit from understanding how local signals and transcriptional programs influence lineage commitment and maturation, helping guide the development of organized adipose tissue.