C-fos Immunohistochemical Detection
C-fos Immunohistochemical Detection - Related Videos
05:44
The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
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Cited by 44 •
2016
Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.
08:07
Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats
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Cited by 24 •
2016
The goal of this study is to identify reward-related distributed brain networks by delineating a reliable immunohistological technique using cellular c-fos activation to measure simultaneous changes in dopamine pathways and terminal sites after novel ingestion of fat and sugar in rats.
01:18
Immunohistochemical Detection of Pathogenic Bacteria in Human Brain Tissue
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2025
To detect these bacteria, take a deparaffinized, rehydrated, bacteria-infected human brain section with exposed protein antigens and inactivated endogenous phosphatase.
01:23
Immunohistochemical Detection of Lewy Bodies in a Diseased Human Brain Tissue Section
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2025
Incubate with hydrogen peroxide solution to inhibit endogenous peroxidases and prevent non-specific staining.
08:37
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
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Cited by 34 •
2016
Here we present a Fluorescence Activated Cell Sorting (FACS) protocol to study molecular alterations in Fos-expressing neuronal ensembles from both fresh and frozen brain tissue. The use of frozen tissue allows FACS isolation of many brain areas over multiple sessions to maximize the use of valuable animal subjects.
FAQs
Nuclear accumulation places c-Fos in a cellular compartment that can be visualized within tissue sections. Because intracellular signaling after stimulation induces c-fos transcription and produces the protein, nuclear labeling connects a detected signal with a recent cellular response. This localization also helps researchers identify which cells, rather than only which tissue regions, responded to a stimulus.
A stimulus first activates intracellular signaling pathways that induce c-fos transcription. The resulting c-Fos protein then accumulates in cell nuclei, where an antibody can recognize it. Immunohistochemical detection converts that molecular response into an enzyme-linked or fluorescent signal, allowing the cellular response to be examined directly in preserved tissue sections.
The distribution of labeled nuclei shows where recent cellular activation occurred within a tissue section. Examining this spatial pattern can reveal whether activation is concentrated in particular regions or cell populations. In biology, that information supports interpretation of how stimulation, behavior, or pharmacological treatment relates to localized patterns of gene expression.
The procedure uses a primary antibody directed against c-Fos to recognize the target protein. A secondary antibody then binds the primary antibody and carries either an enzyme-linked or fluorescent detection signal. This two-antibody arrangement converts target recognition into visible labeling, enabling c-Fos-positive nuclei to be located in tissue sections.
The workflow begins with tissue sections containing cells that may have produced c-Fos after stimulation. A primary anti-c-Fos antibody is applied to recognize the protein, followed by a secondary antibody linked to an enzyme or fluorescent marker. The resulting signal is then examined in the sections to determine the location of labeled nuclei.
This method is useful when researchers need to relate cellular activation to anatomy, behavior, or treatment. It can help map activated neural circuits, compare responses associated with behavioral conditions, and examine effects of pharmacological treatment. By preserving spatial information in tissue, the approach connects molecular activation with the organization of biological systems.