Cell Capture
Cell Capture - Related Videos
29:02
Cell Capture Using a Microfluidic Device
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Cited by 1 •
2007
10:11
Glycopeptide Capture for Cell Surface Proteomics
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Cited by 8 •
2014
Cell surface proteins are biologically important and widely glycosylated. We introduce here a glycopeptide-capture approach to solubilize, enrich, and deglycosylate these proteins for facile LC-MS based proteomic analyses.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
10:24
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
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Cited by 23 •
2015
The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.
10:37
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
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Cited by 37 •
2016
Here we describe protocols to disrupt mammalian cells by solid-state milling at a cryogenic temperature, produce a cell extract from the resulting cell powder, and isolate protein complexes of interest by affinity capture upon antibody-coupled micron-scale paramagnetic beads.
FAQs
Recognition depends on ligands, such as antibodies, binding markers displayed on target-cell membranes. This interaction gives the capture system selectivity within a mixed biological sample, because cells carrying the relevant marker can be retained while unwanted cells are removed. The resulting enrichment helps researchers examine rare populations with less interference from other cells.
Physical capture separates cells according to measurable properties rather than membrane-marker binding. Differences in size, density, or deformability can cause some cells to be retained or isolated from others in the same sample. This approach is useful when physical characteristics provide a practical basis for separation, including situations in which marker-based recognition is not the selected strategy.
Marker-based capture relies on a ligand, commonly an antibody, recognizing a membrane marker, whereas physical capture uses differences in cell size, density, or deformability. The first approach emphasizes biological identity, while the second emphasizes measurable cell characteristics. Choosing between them determines what feature drives separation and influences which cell populations can be recovered for analysis.
After cells are retained, researchers may wash the captured material to remove unwanted cells or sample components. They can then identify or count the retained population, culture it, or release it for molecular analysis. These steps convert the initial separation into a usable biological workflow and allow the same capture process to support different downstream objectives.
The method is valuable when a sample contains a rare population that would be difficult to study among many other cells. Supported applications include diagnostics, immunology, cancer research, stem-cell studies, and single-cell workflows. By reducing interference from unwanted cells, capture can make selected populations more accessible for identification, counting, culture, or molecular investigation.
Captured cells can be identified and counted to characterize the selected population, or maintained in culture when continued study is needed. If released, they become available for molecular analysis. These outcomes allow researchers to move from physical recovery to biological interpretation, linking the presence of particular cells with investigations in disease, immunity, development, or single-cell biology.