Dendritic Cell Culture
Dendritic Cell Culture - Related Videos
06:39
Culture of myeloid dendritic cells from bone marrow precursors
0 Views •
Cited by 22 •
2008
This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.
07:59
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
0 Views •
Cited by 15 •
2012
We present a protocol that permits to view and to quantitatively asses the morphology of the dendritic tree of individual Purkinje cells grown in organotypic cerebellar slice cultures. This protocol is intended to promote studies on the mechanisms of Purkinje cell dendritic development.
09:52
Inducing Dendritic Growth in Cultured Sympathetic Neurons
0 Views •
Cited by 10 •
2012
We describe a protocol for using bone morphogenetic protein-7 (BMP-7) or Matrigel to selectively induce dendritic growth in primary sympathetic neurons dissociated from the superior cervical ganglia (SCG) of perinatal rats.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
0 Views •
Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
11:48
Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
0 Views •
Cited by 69 •
2011
Numerous recent studies have identified mutations in synaptic proteins associated with brain pathologies. Primary cultured cortical neurons offer great flexibility in examining the effects of these disease-associated proteins on dendritic spine morphology and motility.
FAQs
Defined cytokines provide the signals that guide progenitor cells or monocytes toward dendritic-cell differentiation in vitro. Their role is distinct from maturation stimuli, which drive functional changes after the cells have developed. Separating these stages helps investigators examine how developmental state influences surface molecules, secreted cytokines, and T-cell activation.
Maturation can be evaluated through coordinated changes in surface molecules, cytokine secretion, and the capacity to activate T cells. These readouts are useful because they connect cellular state with immune function rather than treating growth alone as the endpoint. In this way, a culture can be assessed for both phenotypic and functional consequences of maturation.
The culture provides a controllable setting for following how dendritic cells capture, process, and present antigens. Researchers can then relate those steps to activation of T cells and to broader immune regulation. Because the cells are maintained under defined in vitro conditions, the model helps examine cellular responses separately from the complexity of a whole biological system.
Two starting populations described for this approach are progenitor cells and monocytes. Investigators expose either population to defined cytokine conditions that support differentiation, then can apply maturation stimuli to examine later functional changes. The choice of starting population therefore forms part of the experimental design when comparing developmental transitions or immune responses in vitro.
A typical workflow begins by maintaining progenitor cells or monocytes under controlled in vitro conditions with cytokines that support differentiation. Once the dendritic-cell population has developed, maturation stimuli can be introduced. Researchers then examine surface molecules, cytokine secretion, antigen handling, or T-cell activation, depending on the biological question.
Dendritic cell culture is useful when investigators need a controllable model of immune regulation or antigen presentation. Applications described for the method include studying innate and adaptive immunity, host responses to pathogens, tumor immunology, and vaccine development. The same system can also help evaluate how conditions affect antigen capture, processing, presentation, and T-cell activation.