Embryonic Larval Zebrafish
Embryonic Larval Zebrafish - Related Videos
05:58
Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations
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Cited by 21 •
2013
Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.
06:00
Forebrain Electrophysiological Recording in Larval Zebrafish
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Cited by 55 •
2013
A simple method to record extracellular field potentials in the larval zebrafish forebrain is described. The method provides a robust in vivo read-out of seizure-like activity. This technique can be used with genetically modified zebrafish larvae carrying epilepsy-related genes or seizures evoked by administration of convulsant drugs.
10:43
Dissection of Larval Zebrafish Gonadal Tissue
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Cited by 6 •
2017
Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance.
06:08
High-resolution Cell Transplantation in Embryonic and Larval Zebrafish
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2024
Here we present a protocol to transplant cells with high spatial and temporal resolution in zebrafish embryos and larvae at any stage between at least 1 and 7 days post fertilization.
02:26
Development of a Zebrafish Larval Infection Model Using Microgavage
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2026
Source: Li, J., et al. Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (2020).This video demonstrates the microgavage procedure, where an anesthetized zebrafish larva is immobilized in agarose and orally injected with a pathogenic bacterium using a tracer-guided needle. The non-invasive delivery mimics natural infection, enabling analysis of bacterial replication, toxin release, and intestinal epithelial damage.
FAQs
Transparency allows investigators to follow developmental events directly in living embryos and larvae rather than relying only on fixed specimens. This makes it possible to connect changes in cells or tissues with later effects on organ formation and nervous-system development. Repeated observation can therefore link cellular behavior to whole-organism outcomes during vertebrate development.
Researchers can alter genes, cells, or environmental conditions and then observe the resulting biological consequences in the developing animal. Combining these interventions with live imaging helps distinguish changes associated with specific mechanisms from broader developmental effects. The approach is useful for relating molecular or cellular events to physiology, disease-related phenotypes, and tissue-level development.
The model supports observations that span multiple biological levels, from cellular mechanisms to organism-wide physiology. Investigators can examine how developmental processes are altered in disease contexts and assess responses to environmental conditions or treatments. This integrated view is valuable because a change detected in cells or tissues can be evaluated alongside its consequences for the developing vertebrate.
Environmental conditions can be manipulated during early development and then evaluated through visible developmental, physiological, or disease-related responses. Because embryos and larvae can be observed while alive, investigators can connect exposure conditions with changes in organ formation, nervous-system development, regeneration, or infection-related outcomes. Careful comparison of conditions helps identify biologically meaningful effects.
A study generally begins with developing embryos or larvae, followed by a planned manipulation of genes, cells, or environmental conditions. Researchers then use live imaging and other observations to monitor development, physiology, disease-related changes, or treatment responses. Results can be interpreted at both cellular and whole-organism levels, depending on the experimental question.
The model is applied to organ formation, nervous-system development, regeneration, toxicology, infection, and drug-response studies. Its external development, transparency, and compatibility with high-throughput designs allow investigators to examine many experimental conditions while retaining access to live developmental outcomes. Findings can provide vertebrate biological context for questions relevant to human health research.