Mouse Model
Mouse Model - Related Videos
06:24
Mouse Models of Periventricular Leukomalacia
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Cited by 28 •
2010
We established mouse models of periventricular leukomalacia (PVL), the predominant brain injury in premature infants characterized by periventricular white matter lesions. Hypoxia/ischemia with/without systemic infection are the primary causes of PVL. Unilateral carotid ligation and hypoxia exposure with/without lipopolysaccharide injection creates PVL-like lesions in P6 mice.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
09:01
Chronic Salmonella Infected Mouse Model
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Cited by 12 •
2010
Establish a chronic bacterial infected mouse model with persistent Salmonella typhimurium colonization in intestine for 27 weeks.
08:43
Orthotopic Mouse Model of Colorectal Cancer
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Cited by 109 •
2007
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
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Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
FAQs
Targeted genetic changes let researchers connect a specific gene with downstream biological effects. By examining altered cells, tissues, and physiological systems, they can identify how gene function contributes to a measurable phenotype, meaning an observable trait or response. This multilevel approach links molecular mechanisms to outcomes at the level of the whole organism.
Naturally occurring traits can reveal biological variation without a planned genetic alteration, whereas targeted changes let researchers examine the effects of a selected gene. Comparing these approaches helps distinguish an association from a consequence of gene alteration. The resulting phenotypes can be evaluated across cells, tissues, and physiological systems, giving biology studies different ways to investigate gene function.
Conserved genes and biological pathways make mice useful for connecting molecular mechanisms with organism-level outcomes, but similarity does not guarantee identical human responses. Species differences can affect how altered genes, tissues, physiological systems, or treatments behave. Consequently, a result may clarify biology in the model while still requiring careful interpretation before it is related to human health.
Researchers may study a naturally occurring trait or introduce a targeted genetic change, then monitor effects in cells, tissues, and physiological systems. Measurements are collected under controlled experimental conditions and compared through observable phenotypes. This workflow helps connect the original biological alteration with changes across levels of organization, rather than relying on a molecular observation alone.
Mouse models are useful for examining gene function, development, immunity, cancer, and infectious disease. Their value comes from allowing researchers to follow how biological changes appear across an organism while maintaining controlled experimental conditions. This makes the approach relevant when a question requires links among genes, cells, tissues, physiological systems, and measurable phenotypes.
Potential treatments and experimental procedures can be tested in mice while researchers monitor measurable biological outcomes. Such studies help connect an intervention with changes in genes, cells, tissues, or physiological systems before findings are considered in relation to human health. However, conserved pathways do not eliminate species differences, so model results require careful interpretation rather than direct assumption of human effects.