Sperm Extraction
Sperm Extraction - Related Videos
10:00
Harvesting Sperm and Artificial Insemination of Mice
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Cited by 17 •
2007
This protocol demonstrates methods for extracting sperm from the testes of males and then inseminating female mice. This procedure is useful when precise time is needed in developmental studies as well as transgenic work.
07:38
Techniques for Imaging Ca2+ Signaling in Human Sperm
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Cited by 17 •
2010
Stimulus-evoked [Ca2+]i signals of individual human sperm are assessed. Motile cells are loaded with Ca2+-sensitive fluorescent dye (AM-ester method) and immobilised in a perfusable chamber. Cells are imaged by time-lapse fluorescence microscopy and stimulated via the perfusing medium. Responses of single cells (or regions) are analysed offline using Excel.
10:02
Two Types of Assays for Detecting Frog Sperm Chemoattraction
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Cited by 5 •
2011
Eggs and the extracellular coatings around eggs frequently release peptides, proteins and small molecules that communicate with sperm to guide them to the egg thereby promoting fertilization. Using frog sperm we describe and compare two classes of assays used to detect sperm chemoattraction – sperm accumulation assays and sperm tracking assays.
05:46
Isolation and In vitro Activation of Caenorhabditis elegans Sperm
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Cited by 26 •
2011
A protocol for isolating and activating spermatids from male C. elegans is described here. Cutting the posterior end of male releases spermatids. The spermatids can be activated by addition of protease.
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
2011
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
FAQs
These laboratory steps concentrate spermatozoa and separate them from other components of a semen sample. Centrifugation brings cells into a concentrated fraction, washing helps prepare that fraction for assessment or further use, and density-based separation supports selection of sperm with useful characteristics, particularly motility. Together, the steps produce a more workable sample for downstream reproductive procedures.
The three measurements describe different aspects of the recovered cell population. Concentration indicates how many sperm are present, motility records movement, and morphology describes cellular form. Considering them together helps a laboratory characterize the preparation and determine what it contains before cells are used in assisted reproductive procedures or examined in studies of sperm function.
Surgical retrieval becomes relevant when semen contains few or no sperm, making semen-based recovery inadequate. In that setting, sperm may be obtained directly from reproductive tissues, including the epididymis or testis. This route provides an alternative source material for laboratory assessment and fertility care when ejaculated samples do not provide enough sperm for planned use.
A laboratory can process the sample by concentrating sperm, washing the recovered cells, and applying density-based separation when appropriate. Technicians then assess concentration, motility, and morphology to characterize the preparation. The resulting information helps determine whether the isolated sperm can support a planned assisted reproductive procedure or should instead be used for investigation of sperm biology.
Recovered sperm can be directed toward assisted reproductive procedures including in vitro fertilization and intracytoplasmic sperm injection. The laboratory assessment performed beforehand supplies information about the number, movement, and form of the available cells. These procedures provide routes for using recovered cells in fertility care, while assessment documents the characteristics of the material being used.
In biology, recovered sperm provide material for studying male infertility, sperm function, and reproductive physiology. The process can also support preservation of genetic material, extending its value beyond immediate sample preparation. Measurements of concentration, motility, and morphology give researchers descriptive information about recovered cells, helping connect laboratory handling with questions about reproductive biology.