Tissue Slicing
Tissue Slicing - Related Videos
03:40
TTC Staining of Rat Brain Tissue Slices: A Staining Procedure to Differentiate Viable and Necrotic Tissue in Tissue Slices After Ischemia-Reperfusion Injury
0 Views •
2025
This video describes the 2,3,5-triphenyl-2H-tetrazolium chloride staining procedure to visualize rat brain tissue slices and differentiate viable and damaged tissue following middle cerebral artery occlusion and reperfusion.
05:02
Cerebellum Dissection and Slice Preparation: A Method to Generate Tissue Slices from Mouse Cerebellum
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2025
This video describes a method to generate tissue slices from the mouse cerebellum. The cerebellar slices can be used for organotypic culturing that provides insights into neurodevelopmental mechanisms, including myelination dynamics.
04:13
Generating and Maintaining Slice Cultures from Human Glioblastoma Tissue
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2025
The video demonstrates the generation and maintenance of organotypic slice cultures from glioblastoma tissue. The isolated tissue is embedded in an agarose matrix, and thin sections are obtained using a vibratome. These sections are then placed on permeable membrane inserts within a microplate and cultured in a slice culture maintenance media for long-term maintenance.
04:58
Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue
0 Views •
2025
This video demonstrates a vibratome-based procedure to generate viable, ultra-thin sections of pig heart. The prepared tissue slices can be cultured and used as model systems for pharmacological testing.
09:31
Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
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Cited by 15 •
2011
In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This physiological approach allows observations of subpopulations and/or individual neurons in a living tissue and is convenient for receptor-ligand identification.
FAQs
Fixation and embedding prepare tissue for sectioning and help preserve cellular and structural relationships during handling. In paraffin preparation, tissue is embedded before a microtome cuts sections; frozen preparation instead supports sectioning with a cryostat. This choice determines the preparation route used before sections are mounted and stained for microscopic analysis.
Section thickness and orientation influence how clearly microscopic structures can be examined. Thickness affects the quality of the resulting analysis, while orientation determines which anatomical relationships appear within a section. Researchers therefore select both parameters carefully when studying cell types, tissue organization, or changes associated with disease or experimental treatment.
The two approaches differ in how tissue is prepared and cut. Paraffin sectioning uses embedded tissue and a microtome, whereas frozen sectioning uses tissue that has been frozen and a cryostat. Both provide sections for mounting and staining, but the selected route affects the preparation process used before microscopic examination.
A typical workflow begins by fixing and embedding tissue in paraffin or freezing the specimen. Researchers then cut sections with a microtome or cryostat, mount the sections, and apply staining when needed. Each stage contributes to producing material suitable for microscopy, while section thickness and orientation help determine the quality and interpretability of the final analysis.
Mounting places the cut section in a form suitable for microscopic examination, while staining makes cellular and structural features available for analysis. These preparations can support identification of cell types and assessment of tissue changes. In immunohistochemistry, stained sections also contribute to examining biological features within their tissue context.
Biologists use tissue slicing in histology, anatomy, pathology, immunohistochemistry, and studies of spatial organization. The resulting sections allow researchers to relate microscopic structure to health, disease, or experimental treatment. This makes the technique useful for examining both normal cellular arrangements and tissue changes while retaining the spatial relationships among structures.